Galectin 3 (GAL3) Recombinant Protein | GAL3 recombinant protein

Recombinant Galectin 3 (GAL3)

Cat. Num. AAA2011553

• Gene Names: Lgals3; gal-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Galectin 3 (GAL3); Recombinant Galectin 3 (GAL3); GAL3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-MADGFSLNDA LAGSGNPNPQ GWPGAWGNQP GAGGYPGASY PGAYPGQAPP GGYPGQAPPS AYPGPTGPSA YPGPTAPGAY PGPTAPGAFP GQPGGPGAYP SAPGAYPSAP GAYPATGPFG APTGPLTVPY DMPLPGGVMP RMLITIIGTV KPNANSITLN FKKGNDIAFH FNPRFNENNR RVIVCNTKQD NNWGREERQS AFPFESGKPF KIQVLVEADH FKVAVNDVHL LQYNHRMKNL REISQLGIIG DITLTSASHA MI
• Sequence Length: 262

• Applicable Applications for GAL3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Ile262 (Accession # P08699) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13929190
• NCBI GeneID: 83781
• NCBI Accession #: NP_114020.1
• NCBI GenBank Nucleotide #: NM_031832.1
• UniProt Accession #: P08699
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: galectin-3
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3
• NCBI Official Symbol: Lgals3
• NCBI Official Synonym Symbols: gal-3
• NCBI Protein Information: galectin-3; CBP 35; lectin L-29; 35 kDa lectin; mac-2 antigen; IgE binding protein; igE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; lectin, galactose binding, soluble 3; lectin galactoside-binding soluble 3 protein
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: Lgals3
• UniProt Synonym Gene Names: Gal-3; CBP 35
• UniProt Entry Name: LEG3_RAT

NCBI Description

lectin molecule that binds IgE; may have role in immune function [RGD, Feb 2006]

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Motility/polarity/chemotaxis; Cell surface; Extracellular matrix

Cellular Component: extracellular matrix; spliceosome; extracellular space; proteinaceous extracellular matrix; cell surface; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: monosaccharide binding; advanced glycation end-product receptor activity; laminin binding; IgE binding; disaccharide binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; positive regulation of serotonin secretion; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; signal transduction; macrophage chemotaxis; monocyte chemotaxis; positive regulation of angiogenesis; epithelial cell differentiation; positive chemotaxis; positive regulation of cell proliferation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development; negative regulation of apoptosis