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Western Blot (WB) (The anti-GALK1 Pab is used in Western blot to detect GALK1 in Y79 cell lysate.)

Rabbit anti-Human, Mouse Galactokinase 1 Polyclonal Antibody

Galactokinase 1, CT (GALK1, Galactokinase, Galactose Kinase, GALK)

Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
Galactokinase 1; Polyclonal Antibody; CT (GALK1; Galactokinase; Galactose Kinase; GALK); Anti -Galactokinase 1; anti-Galactokinase 1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GALK1. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-Galactokinase 1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunogen
Synthetic peptide selected from the C-terminal region of human GALK1 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-GALK1 Pab is used in Western blot to detect GALK1 in Y79 cell lysate.)

Western Blot (WB) (The anti-GALK1 Pab is used in Western blot to detect GALK1 in Y79 cell lysate.)

Western Blot (WB)

(Western blot analysis of GALK1 (arrow) using rabbit polyclonal hGALK1-A360 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GALK1 gene.)

Western Blot (WB) (Western blot analysis of GALK1 (arrow) using rabbit polyclonal hGALK1-A360 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GALK1 gene.)
Related Product Information for anti-Galactokinase 1 antibody
Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts in the adult population. GALK1 sequence shares the greatest level of conservation, 44.5% identity with that from E. coli and 34.6% amino acid identity with the product of the human GALK2 gene.
Product Categories/Family for anti-Galactokinase 1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
galactokinase 1

NCBI Description

Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts during infancy and presenile cataracts in the adult population. [provided by RefSeq, Jul 2008]

Uniprot Description

GALK1: Major enzyme for galactose metabolism. Defects in GALK1 are the cause of galactosemia II (GALCT2). Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses. Belongs to the GHMP kinase family. GalK subfamily.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; Carbohydrate Metabolism - galactose; EC 2.7.1.6

Chromosomal Location of Human Ortholog: 17q24

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: galactokinase activity; galactose binding; ATP binding

Biological Process: galactose catabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; galactose metabolic process; galactitol metabolic process; pathogenesis

Disease: Galactokinase Deficiency

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Product Notes

The Galactokinase 1 (Catalog #AAA625879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Galactokinase 1, CT (GALK1, Galactokinase, Galactose Kinase, GALK) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Galactokinase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the Galactokinase 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galactokinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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