Rabbit anti-Human, Mouse Galactokinase 1 Polyclonal Antibody | anti-GALK1 antibody

Galactokinase 1, CT (GALK1, Galactokinase, Galactose Kinase, GALK) (FITC)

Cat. Num. AAA6477396

• Gene Names: GALK1; GK1; GALK
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Galactokinase 1; Polyclonal Antibody; CT (GALK1; Galactokinase; Galactose Kinase; GALK) (FITC); anti-GALK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GALK1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GALK1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GALK1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-GALK1 Pab is used in Western blot to detect GALK1 in Y79 cell lysate.)

Western Blot (WB)

(Western blot analysis of GALK1 (arrow) using rabbit polyclonal hGALK1-A360. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GALK1 gene.)

Product Categories/Family for anti-GALK1 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4503895
• NCBI GeneID: 2584
• NCBI Accession #: NP_000145.1
• NCBI GenBank Nucleotide #: NM_000154.1
• UniProt Accession #: P51570; B2RC07
• Molecular Weight: 42,272 Da
• NCBI Official Full Name: galactokinase
• NCBI Official Synonym Full Names: galactokinase 1
• NCBI Official Symbol: GALK1
• NCBI Official Synonym Symbols: GK1; GALK
• NCBI Protein Information: galactokinase; galactose kinase
• UniProt Protein Name: Galactokinase
• UniProt Gene Name: GALK1
• UniProt Synonym Gene Names: GALK
• UniProt Entry Name: GALK1_HUMAN

NCBI Description

Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts during infancy and presenile cataracts in the adult population. [provided by RefSeq, Jul 2008]

Uniprot Description

GALK1: Major enzyme for galactose metabolism. Defects in GALK1 are the cause of galactosemia II (GALCT2). Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses. Belongs to the GHMP kinase family. GalK subfamily.

Protein type: Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; EC 2.7.1.6

Chromosomal Location of Human Ortholog: 17q24

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: galactokinase activity; galactose binding; ATP binding

Biological Process: galactose catabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; galactitol metabolic process; galactose metabolic process; pathogenesis

Disease: Galactokinase Deficiency