Rabbit EVER2 Polyclonal Antibody | anti-TMC8 antibody

EVER2 Antibody

Cat. Num. AAA150620

• Gene Names: TMC8; EV2; EVER2; EVIN2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: EVER2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: EVER2; Polyclonal Antibody; EVER2 Antibody; EV2; EVIN2; Transmembrane channel-like protein 8; Epidermodysplasia verruciformis protein 2; transmembrane channel-like 8; anti-TMC8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of EVER2 are known to exist; this antibody will only recognize the larger isoform. EVER2 has no cross-reactivity to EVER1.
• Purity/Purification: EVER2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 726

• Applicable Applications for anti-TMC8 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: EVER2 antibody can be used for detection of EVER2 by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: EVER2 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human EVER2.
• Buffer: EVER2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: EVER2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of EVER2 in rat thymus tissue lysate with EVER2 antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-TMC8 antibody

EVER2 Antibody: Epidermodysplasia verruciformis (EV) is an autosomal recessive genodermatosis associated with a high risk of skin cancers resulting from a high susceptibility to infection by specific human papillomaviruses. Mutations in two homologous genes EVER1 and EVER2 cause the majority of EV cases. These two proteins form a complex and interact with the zinc transporter ZnT-1 in the endoplasmic reticulum. Cells lacking EVER2 accumulated higher levels of zinc in the nucleolus and nucleus compare to those cells with and intact EVER2 gene, indicating that one role of EVER2 is to regulate the intracellular distribution of zinc.

NCBI and Uniprot Product Information

• NCBI GI #: 25527192
• NCBI GeneID: 147138
• UniProt Accession #: Q8IU68; Q2YDC0; Q8IWU7; Q8N358; Q8NF04
• Molecular Weight: 56,132 Da
• NCBI Official Full Name: large EVER2 protein
• NCBI Official Synonym Full Names: transmembrane channel-like 8
• NCBI Official Symbol: TMC8
• NCBI Official Synonym Symbols: EV2; EVER2; EVIN2
• NCBI Protein Information: transmembrane channel-like protein 8; epidermodysplasia verruciformis 2; epidermodysplasia verruciformis protein 2
• UniProt Protein Name: Transmembrane channel-like protein 8
• Protein Family: Transmembrane channel-like protein
• UniProt Gene Name: TMC8
• UniProt Synonym Gene Names: EVER2; EVIN2
• UniProt Entry Name: TMC8_HUMAN

NCBI Description

Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 8 predicted transmembrane domains and 3 leucine zipper motifs. [provided by RefSeq, Jul 2008]

Uniprot Description

TMC8: an endoplasmic reticulum multipass membrane protein. Defects in TMC8 cause Lutz-Lewandowsky epidermodysplasia verruciformis [MIM:226400], a rare skin disease characterized by increased susceptibility to infection by papillomaviruses with an accompanying high risk of skin carcinoma. Three splice-variant isoforms of the human protein have been described.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; cytoplasm; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: negative regulation of protein oligomerization; zinc ion homeostasis; ion transport; regulation of cell growth; negative regulation of protein binding

Disease: Epidermodysplasia Verruciformis