Western Blot (WB)
(GAGE12B Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the GAGE12B antibody detected the GAGE12B protein (arrow).)
Rabbit anti-Human GAGE12B Polyclonal Antibody | anti-GAGE12B antibody
GAGE12B Antibody (N-term)
Gene Names
GAGE12C; GAGE-12B
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GAGE12B; Polyclonal Antibody; GAGE12B Antibody (N-term); G antigen 12B/C/D/E; GAGE-12B; GAGE-12C; GAGE-12D; GAGE-12E; anti-GAGE12B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GAGE12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-49 amino acids from the N-terminal region of human GAGE12B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
21-49
Sequence Length
117
Applicable Applications for anti-GAGE12B antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(GAGE12B Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the GAGE12B antibody detected the GAGE12B protein (arrow).)
Western Blot (WB)
(GAGE12B Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the GAGE12B antibody detected the GAGE12B protein (arrow).)
Immunohistochemistry (IHC)
(GAGE12B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAGE12B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GAGE12B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAGE12B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
References
Gjerstorff, M.F., et al. Tissue Antigens 71(3):187-192(2008)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12925
NCBI Official Full Name
G antigen 12B/C/D/E
NCBI Official Synonym Full Names
G antigen 12C
NCBI Official Symbol
GAGE12C
NCBI Official Synonym Symbols
GAGE-12B
NCBI Protein Information
G antigen 12B/C/D/E
UniProt Protein Name
G antigen 12B/C/D/E
Protein Family
UniProt Gene Name
GAGE12B
UniProt Synonym Gene Names
GAGE-12B; GAGE-12C; GAGE-12D; GAGE-12E
UniProt Entry Name
GG12C_HUMAN
Uniprot Description
GAGE12B/C/D/E: Belongs to the GAGE family
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xp11.23
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Product Notes
The GAGE12B gage12b (Catalog #AAA9206392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-49. The GAGE12B Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAGE12B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the GAGE12B gage12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAGE12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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