Rabbit anti-Human GAGE12B Polyclonal Antibody | anti-GAGE12B antibody

GAGE12B, NT (GAGE12B, G antigen 12B/C/D/E) (PE)

Cat. Num. AAA6300982

• Gene Names: GAGE12B; GAGE-12B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GAGE12B; Polyclonal Antibody; NT (GAGE12B; G antigen 12B/C/D/E) (PE); G antigen 12B/C/D/E; anti-GAGE12B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GAGE12B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GAGE12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-58 amino acids from the N-terminal region of human GAGE12B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GAGE12B Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the GAGE12B antibody detected the GAGE12B protein (arrow).)

Immunohistochemistry (IHC)

(GAGE12B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAGE12B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-GAGE12B antibody

Antibodies; Proteins

References

Gjerstorff, M.F., et al. Tissue Antigens 71(3):187-192(2008). Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 188219778
• NCBI GeneID: 729428
• NCBI Accession #: NP_001120817.1
• NCBI GenBank Nucleotide #: NM_001127345.1
• UniProt Accession #: A1L429; A6NHF1; B7ZL74
• Molecular Weight: 12,925 Da
• NCBI Official Full Name: G antigen 12B/C/D/E
• NCBI Official Synonym Full Names: G antigen 12B
• NCBI Official Symbol: GAGE12B
• NCBI Official Synonym Symbols: GAGE-12B
• NCBI Protein Information: G antigen 12B/C/D/E; g antigen 12C/D/E
• UniProt Protein Name: G antigen 12B/C/D/E
• Protein Family: G antigen
• UniProt Gene Name: GAGE12B
• UniProt Synonym Gene Names: GAGE-12B; GAGE-12C; GAGE-12D; GAGE-12E

Uniprot Description

MiscellaneousThis gene belongs to a multigene family expressed in a large variety of tumors whereas in normal tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells.