Human ITIH3 (Inter-alpha-trypsin inhibitor heavy chain H3) ELISA Kit | ITIH3 elisa kit

Human ITIH3 (Inter-alpha-trypsin inhibitor heavy chain H3) ELISA Kit

Cat. Num. AAA7608170

• Gene Names: ITIH3; H3P; SHAP; ITI-HC3
• Reactivity: Human
• Synonyms: ITIH3 (Inter-alpha-trypsin inhibitor heavy chain H3); Human ITIH3 (Inter-alpha-trypsin inhibitor heavy chain H3) ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H3; ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; Serum-derived hyaluronan-associated protein; SHAP; ITIH3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITIH3. No significant cross-reactivity or interference between ITIH3 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITIH3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 133925809
• NCBI GeneID: 3699
• NCBI Accession #: NP_002208.3
• NCBI GenBank Nucleotide #: NM_002217.3
• UniProt Accession #: Q06033; Q3B7H5; Q53F06; Q6LAM2; Q99085
• Molecular Weight: 99,328 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H3 preproprotein
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 3
• NCBI Official Symbol: ITIH3
• NCBI Official Synonym Symbols: H3P; SHAP; ITI-HC3
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H3
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H3
• Protein Family: Inter-alpha-trypsin inhibitor
• UniProt Gene Name: ITIH3
• UniProt Synonym Gene Names: ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP

NCBI Description

This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3. [provided by RefSeq, Jul 2015]

Uniprot Description

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.