Rabbit anti-Human GADD45A Polyclonal Antibody | anti-GADD45A antibody

GADD45A, CT (Growth Arrest And DNA-damage-inducible Protein GADD45 alpha, DNA-damage-inducible Transcript 1, DDIT-1, DDIT1, GADD45) (PE)

Cat. Num. AAA6477370

• Gene Names: GADD45A; DDIT1; GADD45
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GADD45A; Polyclonal Antibody; CT (Growth Arrest And DNA-damage-inducible Protein GADD45 alpha; DNA-damage-inducible Transcript 1; DDIT-1; DDIT1; GADD45) (PE); anti-GADD45A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GADD45A.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GADD45A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GADD45A (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GADD45A Antibody (C-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(GADD45A Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.)

Product Categories/Family for anti-GADD45A antibody

Antibodies; Apoptosis

References

1. Li, L.S., J. Biol. Chem. 283(31), 21394-21403 (2008). 2. Al-Romaih, K., Neoplasia 10(5), 471-480 (2008). 3. Zhu, Q.S., Cancer Res. 68(8), 2895-2903 (2008). 4. Kearsey, J.M., Oncogene 11(9), 1675-1683 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4503287
• NCBI GeneID: 1647
• NCBI Accession #: NP_001915
• NCBI GenBank Nucleotide #: NM_001924.3
• UniProt Accession #: P24522; Q5TCA7; Q5TCA8
• Molecular Weight: 19.4 kDa (173aa), confirmed by MALDI-TOF
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 alpha isoform 1
• NCBI Official Synonym Full Names: growth arrest and DNA damage inducible alpha
• NCBI Official Symbol: GADD45A
• NCBI Official Synonym Symbols: DDIT1; GADD45
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 alpha
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
• Protein Family: Growth arrest and DNA damage-inducible protein
• UniProt Gene Name: GADD45A
• UniProt Synonym Gene Names: DDIT1; GADD45; DDIT-1
• UniProt Entry Name: GA45A_HUMAN

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; DNA repair, damage; Nuclear receptor co-regulator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; centrosome cycle; activation of MAPKKK activity; DNA damage response, signal transduction; negative regulation of protein kinase activity; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity; DNA repair; cell cycle arrest