anti-Human gAcrp30 Polyclonal Antibody
Anti - gAcrp30 (Globular Adipocyte Complement-related Protein of 30kD, apm-1, Adipolean)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
gAcrp30; Polyclonal Antibody; Anti - gAcrp30 (Globular Adipocyte Complement-related Protein of 30kD; apm-1; Adipolean); gAcrp30 (Globular Adipocyte Complement-related Protein of 30kD; anti-gAcrp30 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human gACRP30.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, 0.05% sodium azide, 40% glycerol.
Supplied as a liquid, 0.05% sodium azide, 40% glycerol.
Applicable Applications for anti-gAcrp30 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blot. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000
ELISA: 1:10,000-1:50,000. Coat plates with 10-100ng of Control Peptide/well.
Optimal dilutions to be determined by researcher.
Dilution: Western Blot: 1:1000-1:5000
ELISA: 1:10,000-1:50,000. Coat plates with 10-100ng of Control Peptide/well.
Optimal dilutions to be determined by researcher.
Positive Control
Western Blot Control: G1016-02
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-gAcrp30 antibody
Acrp30 (adipocyte complement-related protein of 30kD). It is also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript. It was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans. Low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased Acrp30 levels. Acrp30 (mouse 247aa, rat human 244aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa), followed by a 27aa unique region, and then by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus. Structurally, but at the sequence level, Acrp30 resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins). Acrp30 is proteolytically cleaved at 104aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. A marked reduction in plasma triglycerides, glucose, and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle. Full length Acrp30 was less potent than gAcrp30. Therefore, gAcrp30 may open new avenues to control obesity.
Product Categories/Family for anti-gAcrp30 antibody
References
• 1. Scherer, P.E., et al., JBC 270: 26746 (1995).
• 2. Hu, E., et al., JBC 271: 10697 (1996).
• 3. Das, K., et al., BBRC 280: 1120 (2001).
• 4. Fruebis, J., et al., PNAS 98: 2005 (2001).
• 5. Maeda, K., et al., BBRC 221: 286 (1996).
• 6. Schaffler, A., et al., BBA 1399: 187 (1998).
• 7. Schaffler, A., et al., BBRC 260: 416 (1999).
• 2. Hu, E., et al., JBC 271: 10697 (1996).
• 3. Das, K., et al., BBRC 280: 1120 (2001).
• 4. Fruebis, J., et al., PNAS 98: 2005 (2001).
• 5. Maeda, K., et al., BBRC 221: 286 (1996).
• 6. Schaffler, A., et al., BBA 1399: 187 (1998).
• 7. Schaffler, A., et al., BBRC 260: 416 (1999).
Similar Products
Product Notes
The gAcrp30 (Catalog #AAA614136) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - gAcrp30 (Globular Adipocyte Complement-related Protein of 30kD, apm-1, Adipolean) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's gAcrp30 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blot. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-1:50,000. Coat plates with 10-100ng of Control Peptide/well. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the gAcrp30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "gAcrp30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
