SDS-Page
(Effect of Adiponectin (044489) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. |C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. |1: 25ug/ml of Recombinant Adiponectin (rat)|2: 25ug/ml of a DYKDDDDK-tagged control protein.|3: phosphate-buffered saline.)
Adiponectin, Rat Active Protein | Adipoq active protein
Adiponectin, Recombinant, Rat (ACRP30, AdipoQ, apM1, GBP28, Adipocyte Complement Related Protein of 30kD)
Gene Names
Adipoq; Acdc; Acrp30
Purity
Highly Purified>90% (SDS-PAGE)
Synonyms
Adiponectin; Rat; Recombinant; Rat (ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kD); Adipoq active protein
Host
Rat, HEK 293 cells
Purity/Purification
Highly Purified
>90% (SDS-PAGE)
>90% (SDS-PAGE)
Form/Format
Supplied as a liquid from a 0.2um-filtered solution in 30mM TRIS-Cl, pH 8.5.
Sequence
Rat adiponectin (aa 16-244) is fused at the N-terminus to a DYKDDDDK-tag.
Biological Activity
Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.
Preparation and Storage
Short-term Storage: +4 degree C
Long-term Storage: -20 degree C
Working aliquots are stable for up to 3 months when stored at -20 degree C.
Long-term Storage: -20 degree C
Working aliquots are stable for up to 3 months when stored at -20 degree C.
SDS-Page
(Effect of Adiponectin (044489) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. |C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. |1: 25ug/ml of Recombinant Adiponectin (rat)|2: 25ug/ml of a DYKDDDDK-tagged control protein.|3: phosphate-buffered saline.)
SDS-Page
(Effect of Adiponectin (044489) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. |C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. |1: 25ug/ml of Recombinant Adiponectin (rat)|2: 25ug/ml of a DYKDDDDK-tagged control protein.|3: phosphate-buffered saline.)
Related Product Information for Adipoq active protein
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
Product Categories/Family for Adipoq active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~30kD (SDS-PAGE)
NCBI Official Full Name
adiponectin
NCBI Official Synonym Full Names
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol
Adipoq
NCBI Official Synonym Symbols
Acdc; Acrp30
NCBI Protein Information
adiponectin; adipocyte complement related protein of 30 kDa
Protein Family
NCBI Description
adipocyte complement-related factor that plays a role in obesity-related insulin resistance [RGD, Feb 2006]
Research Articles on Adipoq
Similar Products
Product Notes
The Adipoq (Catalog #AAA636381) is an Active Protein produced from Rat, HEK 293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Rat adiponecti n (aa 16-244) is fused at the N-terminus to a DYKDDDDK-t ag. It is sometimes possible for the material contained within the vial of "Adiponectin, Rat, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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