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Western Blot (WB) (Western Blot analysis of Hela Whole Cell Lysate using IL3RA Monoclonal Antibody at dilution of 1:500.)

Rabbit anti-Human IL3RA  Monoclonal Antibody | anti-IL3RA  antibody

Rabbit anti Human IL3RA  Monoclonal Antibody

Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity purification
Synonyms
IL3RA ; Monoclonal Antibody; Rabbit anti Human IL3RA  Monoclonal Antibody; Anti-CD123 Antibody; Anti-hIL-3Ra Antibody; Anti-IL3R Antibody; Anti-IL3RAY Antibody; Anti-IL3RX Antibody; Anti-IL3RY Antibody; anti-IL3RA  antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
0.2 um filtered solution in PBS.
Applicable Applications for anti-IL3RA  antibody
Western Blot (WB), ELISA (EIA)
Application Notes
IHC-P: 1:500-1:1000
ELISA: 1:5000-1:10000
Immunogen
Recombinant Human IL3RA protein
Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Hela Whole Cell Lysate using IL3RA Monoclonal Antibody at dilution of 1:500.)

Western Blot (WB) (Western Blot analysis of Hela Whole Cell Lysate using IL3RA Monoclonal Antibody at dilution of 1:500.)
Related Product Information for anti-IL3RA  antibody
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and/or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 43 kDa
Observed MW: 38 kDa
NCBI Official Full Name
interleukin-3 receptor subunit alpha isoform 2
NCBI Official Synonym Full Names
interleukin 3 receptor subunit alpha
NCBI Official Symbol
IL3RA
NCBI Official Synonym Symbols
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
NCBI Protein Information
interleukin-3 receptor subunit alpha
UniProt Protein Name
Interleukin-3 receptor subunit alpha
UniProt Gene Name
IL3RA
UniProt Synonym Gene Names
IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
UniProt Entry Name
IL3RA_HUMAN

NCBI Description

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]

Uniprot Description

IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3 or Yp11.3

Cellular Component: plasma membrane

Molecular Function: interleukin-3 receptor activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: MAPKKK cascade

Research Articles on IL3RA 

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Product Notes

The IL3RA  il3ra (Catalog #AAA2544022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human IL3RA  Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL3RA  can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). IHC-P: 1:500-1:1000 ELISA: 1:5000-1:10000. Researchers should empirically determine the suitability of the IL3RA  il3ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL3RA , Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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