Western Blot (WB)
((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ARPC1A Polyclonal Antibody | anti-ARPC1A antibody
Goat anti-ARPC1A Antibody
Western Blot: Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 41.6kDa according to NP_006400.2). Approx 37kDa band observed in fetal Mouse Brain and adult Rat Brain lysates (calculated MW of 41.6kDa according to Mouse NP_062741.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
ARPC1A: Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Belongs to the WD repeat ARPC1 family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: actin cytoskeleton; Arp2/3 protein complex; cytosol
Molecular Function: actin binding; actin filament binding
Biological Process: actin cytoskeleton organization and biogenesis; ephrin receptor signaling pathway
Research Articles on ARPC1A
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Product Notes
The ARPC1A arpc1a (Catalog #AAA422740) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ARPC1A Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ARPC1A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 41.6kDa according to NP_006400.2). Approx 37kDa band observed in fetal Mouse Brain and adult Rat Brain lysates (calculated MW of 41.6kDa according to Mouse NP_062741.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the ARPC1A arpc1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DFKTLESSIQ GLR. It is sometimes possible for the material contained within the vial of "ARPC1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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