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Western Blot (WB) (Western Blot analysis of extracts of NCL-H460 cells, using LSM2 antibody.)

Rabbit anti-Human LSM2 Polyclonal Antibody | anti-LSM2 antibody

LSM2 Polyclonal Antibody

Gene Names
LSM2; G7B; snRNP; C6orf28; YBL026W
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
LSM2; Polyclonal Antibody; LSM2 Polyclonal Antibody; G7B; snRNP; C6orf28; YBL026W; anti-LSM2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
LSM2 polyclonal antibody detects endogenous levels of LSM2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
95
Applicable Applications for anti-LSM2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant protein of human LSM2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of NCL-H460 cells, using LSM2 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of NCL-H460 cells, using LSM2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric cancer using LSM2 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human gastric cancer using LSM2 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-LSM2 antibody
Sm and Sm-like (LSm) proteinsform donutshaped heptamericcomplexesthat are involved in varioussteps of RNAmetabolism.LSmproteinsfacilitate RNA protein interactions and structuralchangesthat are required during ribosomalsubunit assembly. The U6 snRNA-associated Sm-like protein LSm2 is also known assmall nuclear ribonucleoprotein D homolog SNP3.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 11kDa
NCBI Official Full Name
snRNP
NCBI Official Synonym Full Names
LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
NCBI Official Symbol
LSM2
NCBI Official Synonym Symbols
G7B; snRNP; C6orf28; YBL026W
NCBI Protein Information
U6 snRNA-associated Sm-like protein LSm2
UniProt Protein Name
U6 snRNA-associated Sm-like protein LSm2
Protein Family
UniProt Gene Name
LSM2
UniProt Synonym Gene Names
C6orf28; G7B
UniProt Entry Name
LSM2_HUMAN

NCBI Description

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19. [provided by RefSeq, Nov 2011]

Uniprot Description

LSM2: Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing. Identified in the spliceosome C complex. LSm subunits form a heteromer with a doughnut shape. Belongs to the snRNP Sm proteins family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; Ral GTPase binding; protein kinase binding; U6 snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA catabolic process, deadenylation-dependent decay

Research Articles on LSM2

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Product Notes

The LSM2 lsm2 (Catalog #AAA3008988) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSM2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LSM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the LSM2 lsm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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