Rabbit GABPB2 Polyclonal Antibody | anti-GABPB1 antibody

GABPB2 antibody - C-terminal region

Cat. Num. AAA3224672

• Gene Names: GABPB1; E4TF1; GABPB; BABPB2; E4TF1B; GABPB2; NRF2B1; NRF2B2; GABPB-1; E4TF1-47; E4TF1-53
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: GABPB2; Polyclonal Antibody; GABPB2 antibody - C-terminal region; anti-GABPB1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: PDGQQVLTVPATDIAEETVISEEPPAKRQCIEIIENRVESAEIEEREALQ
• Sequence Length: 395

• Applicable Applications for anti-GABPB1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Homology: Chicken: 92%
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GABPB2
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Lung)

Immunohistochemistry (IHC)

(Human Spleen)

Western Blot (WB)

(Lanes:1. 40 ug human HK2 cell (kidney proximal tubular cell line) lysate2. 40 ug H2O2 treated human HK2 Cell lysate3. 40 ug H2O2 treated human HK2 Cell lysate4. 40 ug H2O2 treated human HK2 Cell lysate5. 40 ug H2O2 treated human HK2 Cell lysate6. 40 ug H2O2 treated human HK2 Cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Goat anti-Rabbit HRPSecondary Antibody Dilution:1:2000Gene Name:GABPB2Submitted by:The University of Queensland, School of Medicine, Centre for Kidney Disease Research)

Western Blot (WB)

(WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysateGABPB1 is supported by BioGPS gene expression data to be expressed in HepG2)

Related Product Information for anti-GABPB1 antibody

This is a rabbit polyclonal antibody against GABPB2. It was validated on Western Blot and immunohistochemistry

Target Description: GABPB2 is the GA-binding protein transcription factor, beta subunit. It forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex.

Product Categories/Family for anti-GABPB1 antibody

Polyclonal; Transcription Factor; Chromatin & Nuclear Signaling; Various; DNA/RNA/Protein Interactions; Immunohistochemistry; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 8051593
• NCBI GeneID: 2553
• NCBI Accession #: NP_005245
• NCBI GenBank Nucleotide #: NM_005254
• UniProt Accession #: Q06547
• Molecular Weight: 42kDa
• NCBI Official Full Name: GA-binding protein subunit beta-1 isoform beta 1
• NCBI Official Synonym Full Names: GA binding protein transcription factor subunit beta 1
• NCBI Official Symbol: GABPB1
• NCBI Official Synonym Symbols: E4TF1; GABPB; BABPB2; E4TF1B; GABPB2; NRF2B1; NRF2B2; GABPB-1; E4TF1-47; E4TF1-53
• NCBI Protein Information: GA-binding protein subunit beta-1
• UniProt Protein Name: GA-binding protein subunit beta-1
• Protein Family: GA-binding protein
• UniProt Gene Name: GABPB1
• UniProt Synonym Gene Names: E4TF1B; GABPB; GABPB2; GABP subunit beta-1; GABPB-1; GABPB-2
• UniProt Entry Name: GABP1_HUMAN

NCBI Description

This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GABPB1: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; transcription, DNA-dependent; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter