Rabbit Ga15/16 Polyclonal Antibody | anti-Ga15/16 antibody

Ga15 / 16 Antibody

Cat. Num. AAA840543

• Gene Names: GNA15; GNA16
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Ga15/16; Polyclonal Antibody; Ga15 / 16 Antibody; G alpha binding protein; anti-Ga15/16 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-Galpha15 polyclonal antibody in PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 374

• Applicable Applications for anti-Ga15/16 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml) and in Immunohistochemistry (5-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects Galpha15 in samples from human, mouse, rat, and rabbit origins. It also detects Galpha16 from samples of human origin. Reactivity to other species has not been determined.
• Immunogen: Synthetic peptide surrounding amino acid 356of human G 15
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Ga15/16 antibody

Background: Galpha16 is the only heterotrimeric G protein with a restricted expression pattern in hematopoietic cells. Differentiation of promyelocytic cells leads to decreased expression of Galpha16. The G protein is known to couple a large number of G protein-coupled receptors to PLC-beta and can lead to production of the secondary messengers diacylglycerol and inositol phosphates. Targeted deletion of Galpha15, a mouse orthologue of human Galpha16, causes reduced C5alpha receptor signaling. Galpha16 serves as a marker, in addition to CD34, for hematopoietic progenitor cells.

NCBI and Uniprot Product Information

• NCBI GI #: 3289977
• NCBI GeneID: 2769
• Molecular Weight: 43,568 Da
• NCBI Official Full Name: GNA15
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha 15 (Gq class)
• NCBI Official Symbol: GNA15
• NCBI Official Synonym Symbols: GNA16
• NCBI Protein Information: guanine nucleotide-binding protein subunit alpha-15
• UniProt Protein Name: Guanine nucleotide-binding protein subunit alpha-15
• UniProt Gene Name: GNA15
• UniProt Synonym Gene Names: GNA16; G alpha-15; G-protein subunit alpha-15; G alpha-16; G-protein subunit alpha-16
• UniProt Entry Name: GNA15_HUMAN

Uniprot Description

G-alpha 15: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Belongs to the G-alpha family. G(q) subfamily.

Protein type: G protein, heterotrimeric alpha G(q); G protein; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: plasma membrane; heterotrimeric G-protein complex

Molecular Function: GTPase activity; signal transducer activity; GTP binding; G-protein-coupled receptor binding; metal ion binding; G-protein beta/gamma-subunit binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; platelet activation; elevation of cytosolic calcium ion concentration; phospholipase C activation; metabolic process; dopamine receptor, phospholipase C activating pathway; blood coagulation; muscarinic acetylcholine receptor, phospholipase C activating pathway