Rabbit anti-Human G6PC Polyclonal Antibody | anti-G6PC antibody

G6PC, ID (G6PC, G6PT, Glucose-6-phosphatase, Glucose-6-phosphatase alpha)

Cat. Num. AAA6007920

• Gene Names: G6PC; G6PT; GSD1; G6PC1; GSD1a
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A affinity chromatography.
• Synonyms: G6PC; Polyclonal Antibody; ID (G6PC; G6PT; Glucose-6-phosphatase; Glucose-6-phosphatase alpha); Anti -G6PC; anti-G6PC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human G6PC
• Purity/Purification: Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-G6PC antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC: 1:50-1:100; FC: 1:10-1:50; ; Optimal dilutions to be determined by the researcher.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment ofresidues within aa123-149 in the central region of human G6PC.
• Grade: Affinity Purified
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of K562 cell line lysates (35ug/lane) using MBS6007920. G6PC (arrow) was detected using MBS6007920.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6007920 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6007920 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS6007920 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-G6PC antibody

Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I.

Product Categories/Family for anti-G6PC antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 393537072
• NCBI GeneID: 2538
• NCBI Accession #: NP_001257326.1
• NCBI GenBank Nucleotide #: NM_001270397.1
• UniProt Accession #: P35575; A1L4C0; B4E1C3; K7EL82
• NCBI Official Full Name: glucose-6-phosphatase isoform 2
• NCBI Official Synonym Full Names: glucose-6-phosphatase, catalytic subunit
• NCBI Official Symbol: G6PC
• NCBI Official Synonym Symbols: G6PT; GSD1; G6PC1; GSD1a
• NCBI Protein Information: glucose-6-phosphatase; G6Pase; G-6-Pase; G6Pase-alpha; glucose-6-phosphatase alpha
• UniProt Protein Name: Glucose-6-phosphatase
• Protein Family: Glucose-6-phosphatase
• UniProt Gene Name: G6PC
• UniProt Synonym Gene Names: G6PT; G-6-Pase; G6Pase
• UniProt Entry Name: G6PC_HUMAN

NCBI Description

Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

Uniprot Description

G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Endoplasmic reticulum; Membrane protein, multi-pass; Carbohydrate Metabolism - galactose; Phosphatase (non-protein); Membrane protein, integral; Transporter; Transporter, SLC family; Carbohydrate Metabolism - starch and sucrose; EC 3.1.3.9

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: endoplasmic reticulum membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: glucose-6-phosphatase activity; phosphotransferase activity, alcohol group as acceptor; phosphate binding

Biological Process: response to food; steroid metabolic process; glycogen metabolic process; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; glucose homeostasis; gluconeogenesis; cholesterol homeostasis; triacylglycerol metabolic process; urate metabolic process; glucose-6-phosphate transport; regulation of gene expression; hexose transport; carbohydrate metabolic process; transmembrane transport

Disease: Glycogen Storage Disease Ia