Rabbit G-Protein Gi alpha1/alpha2 Polyclonal Antibody | anti-GNAI1 antibody

G-Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2) (AP)

Cat. Num. AAA6478770

• Gene Names: GNAI1; Gi
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: G-Protein Gi alpha1/alpha2; Polyclonal Antibody; G-Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein); alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein); alpha inhibiting activity polypeptide 2) (AP); anti-GNAI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human G protein subunit alphai1 and alpha2. Species Crossreactivity: mouse, rat and bovine
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-GNAI1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC: Rat cerebellum; IHC-P: Paraffin sections require HIER with citrate buffer, pH 6.0; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa345-354 of Gi alpha1 and 346-355 of Gi alpha2 (NM_002069.5).
• Conjugate: AP
• Positive Control: Western Blot: L6 Cell Lysate
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Representative images from a previous lot. Optimal staining of MBS620758 with Citrate pH 6.0 epitope retrieval: rat cerebellum Representative staining pattern/morphology in rat cerebellum usingMBS620758. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of MBS620758 was diluted to 1:100, using IHC-Select Detection with HRP-DAB)

Testing Data

(L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates G8599-05A(~40kD).)

Testing Data

(Representative image from a previous lot. Huvec lysate (Lane 1), A431 lysate (Lane 2), L6 lysate (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500) Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates G protein Gialpha 1 and 2 (~40kD).)

Product Categories/Family for anti-GNAI1 antibody

Antibodies; G Proteins

References

1. Li X, et al. (1995) J.Neurochem. 64, 1107-1117.

NCBI and Uniprot Product Information

• NCBI GI #: 374081863
• NCBI GeneID: 2770
• NCBI Accession #: NP_001243343.1
• NCBI GenBank Nucleotide #: NM_002069.5
• UniProt Accession #: P63096; P04898; P11015; P31871; Q5U074; Q8TAN5; Q9UGA4; A8KA88; B4E2V1; C9J3A4
• Molecular Weight: 34,775 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(i) subunit alpha-1 isoform 2
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
• NCBI Official Symbol: GNAI1
• NCBI Official Synonym Symbols: Gi
• NCBI Protein Information: guanine nucleotide-binding protein G(i) subunit alpha-1; guanine nucleotide-binding protein G(i) subunit alpha-1; Gi1 protein alpha subunit; adenylate cyclase-inhibiting G alpha protein
• UniProt Protein Name: Guanine nucleotide-binding protein G(i) subunit alpha-1
• UniProt Gene Name: GNAI1
• UniProt Entry Name: GNAI1_HUMAN

NCBI Description

Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

G-alpha i1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Interacts with GPSM1. Interacts with RGS12. Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains); the interaction occurs in the centrosomes. Interacts (GDP-bound form) with RIC8A (via C-terminus). Interacts with DRD2. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein, heterotrimeric; G protein, heterotrimeric alpha G((i/o/t/z)); G protein

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: centrosome; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; midbody; nucleus

Molecular Function: GTPase activity; signal transducer activity; protein binding; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; response to peptide hormone stimulus; platelet activation; synaptic transmission; cell division; metabolic process; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; cell cycle