Rabbit G protein alpha S/GNAS Polyclonal Antibody | anti-GNAS antibody

Anti-G protein alpha S/GNAS Antibody, Rabbit Polyclonal

Cat. Num. AAA8100712

• Gene Names: GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45
• Applications: Western Blot, Immunoprecipitation
• Purity: ntigen Affinity Chromatography
• Synonyms: G protein alpha S/GNAS; Polyclonal Antibody; Anti-G protein alpha S/GNAS Antibody; Rabbit Polyclonal; GNAS Antibody; Rabbit PAb; Antigen Affinity Purified; GNAS complex locus; Anti-AHO Antibody; Anti-C20orf45 Antibody; Anti-GNAS1 Antibody; Anti-GPSA Antibody; Anti-GSA Antibody; Anti-GSP Antibody; Anti-NESP Antibody; Anti-PHP1A Antibody; Anti-PHP1B Antibody; Anti-PHP1C Antibody; Anti-POH Antibody; Anti-SgVI Antibody; anti-GNAS antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GNAS
• Purity/Purification: ntigen Affinity Chromatography
• Form/Format: 0.2um filtered solution in PBS

• Applicable Applications for anti-GNAS antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-4uL/mg of lysate; Please Note: Optimal concentrations/dilutions should be determined by the end users.
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human GNAS
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human GNAS, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles. Sodium Azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.

Immunoprecipitation (IP)

(GNAS was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-GNAS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GNAS rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)

Western Blot (WB)

(Anti-GNAS rabbit polyclonal antibody at 1:500 dilution.Lane A: MCF7 Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:55 kDa)

Related Product Information for anti-GNAS antibody

GNAS (GNAS Complex Locus) is a Protein Coding gene. GNAS is a complex imprinted gene encoding the alpha-subunit of the stimulatory heterotrimeric G protein (Gs alpha), which belongs to the G-alpha family. Inactivating mutations in Gsalpha-coding GNAS exons are responsible for Albright's hereditary osteodystrophy (AHO), which refers to a constellation of physical and developmental disorders including obesity, short stature, brachydactyly, cognitive impairment, and heterotopic ossification. GNAS mediates receptor-stimulated cAMP signaling, which integrates diverse environmental cues with intracellular responses. GNAS mutations have been described in mucinous and non-mucinous epithelial neoplasms of the appendix, pancreas, and colon, with hotspot GNAS mutations found in up to two-thirds of pancreatic intraductal papillary mucinous neoplasms.

NCBI and Uniprot Product Information

• NCBI GI #: 117938768
• NCBI GeneID: 2778
• NCBI Accession #: NP_001070958.1
• NCBI GenBank Nucleotide #: NM_001077490.2
• UniProt Accession #: P84996; A2A2S4; O95467; P63092; Q5JWF2
• Molecular Weight: 67,948 Da
• NCBI Official Full Name: protein ALEX Alex
• NCBI Official Synonym Full Names: GNAS complex locus
• NCBI Official Symbol: GNAS
• NCBI Official Synonym Symbols: AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45
• NCBI Protein Information: protein ALEX; protein GNAS; protein SCG6 (secretogranin VI)
• UniProt Protein Name: Protein ALEX
• Protein Family: Protein
• UniProt Gene Name: GNAS

NCBI Description

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

Uniprot Description

May inhibit the adenylyl cyclase-stimulating activity of guanine nucleotide-binding protein G(s) subunit alpha which is produced from the same locus in a different open reading frame.