Rabbit G-CSF Polyclonal Antibody | anti-G-CSF antibody

RABBIT ANTI HUMAN G-CSF:Biotin

Cat. Num. AAA220159

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Applications: ELISA, Western Blot
• Synonyms: G-CSF; Polyclonal Antibody; RABBIT ANTI HUMAN G-CSF:Biotin; anti-G-CSF antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Biotin; Purified IgG conjugated to Biotin - lyophilised
• Concentration: IgG concentration 0.1 mg/ml after reconstitution. (varies by lot)
• Sequence Length: 171

• Applicable Applications for anti-G-CSF antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: This biotinylated human G-CSF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human G-CSF antibody as the capture reagent and recombinant human G-CSF as the standard.; ELISA: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
• Immunogen: Recombinant human G-CSF; Buffer Solution
• Antiserum Preparation: Antisera to human G-CSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human angiosarcoma with Rabbit anti Human G-CSF (MBS221336) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Testing Data #2

(Staining of formalin fixed, paraffin embedded human angiosarcoma with Rabbit anti Human G-CSF (MBS221336) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-G-CSF antibody

Rabbit anti Human G-CSF antibody recognizes human G-CSF (Granulocyte colony-stimulating factor), a 19.6kDa haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage. The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 ug/ml of this antibody is required.

NCBI and Uniprot Product Information

• NCBI GI #: 4503079
• NCBI GeneID: 1440
• NCBI Accession #: NP_000750.1
• NCBI GenBank Nucleotide #: NM_000759.3
• UniProt Accession #: P09919; A8MXR7
• Molecular Weight: 18,434 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor; filgrastim; granulocyte-colony stimulating factor; lenograstim; pluripoietin
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein binding; positive regulation of actin filament polymerization; positive regulation of myeloid cell differentiation; granulocyte differentiation; cytokine and chemokine mediated signaling pathway; multicellular organismal development; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of peptidyl-serine phosphorylation