Rabbit anti-Human, Mouse FZD9 Polyclonal Antibody | anti-FZD9 antibody

FZD9 Antibody

Cat. Num. AAA7048213

• Gene Names: FZD9; FZD3; CD349
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: FZD9; Polyclonal Antibody; FZD9 Antibody; Frizzled-9; Fz-9; hFz9; FzE6; CD349; FZD3; (CD Antigen CD349); anti-FZD9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-FZD9 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:5000; IHC: 1:500 - 1:1000
• Immunogen: Recombinant Human Frizzled-9 protein (23-229AA)
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Species: Homo sapiens (Human)
• Research Area: Neuroscience
• Target Names: FZD9
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse liver tissue,Mouse kidney tissueAll lanes: FZD9 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65 kDaObserved band size: 65 kDa)

Immunohistochemistry (IHC)

(IHC image of MBS7048213 diluted at 1:500 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Related Product Information for anti-FZD9 antibody

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the betacatenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 4503835
• NCBI GeneID: 8326
• NCBI Accession #: NP_003499.1
• NCBI GenBank Nucleotide #: NM_003508.2
• UniProt Accession #: O00144
• NCBI Official Full Name: frizzled-9
• NCBI Official Synonym Full Names: frizzled class receptor 9
• NCBI Official Symbol: FZD9
• NCBI Official Synonym Symbols: FZD3; CD349
• NCBI Protein Information: frizzled-9
• UniProt Protein Name: Frizzled-9
• Protein Family: Frizzled
• UniProt Gene Name: FZD9
• UniProt Synonym Gene Names: FZD3; Fz-9; hFz9
• UniProt Entry Name: FZD9_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD9: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum membrane; Golgi apparatus; mitochondrial membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein heterodimerization activity; protein homodimerization activity; Wnt receptor activity; Wnt-protein binding

Biological Process: cytosolic calcium ion homeostasis; negative regulation of mitochondrial depolarization; negative regulation of neuron apoptosis; nervous system development; ossification; positive regulation of apoptosis; positive regulation of bone mineralization; release of cytochrome c from mitochondria; Wnt receptor signaling pathway through beta-catenin