Goat FYB Polyclonal Antibody | anti-FYB antibody

Goat anti-FYB Antibody

Cat. Num. AAA423056

• Gene Names: FYB; ADAP; PRO0823; SLAP130; SLAP-130
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FYB; Polyclonal Antibody; Goat anti-FYB Antibody; FYN binding protein (FYB-120/130); ADAP; PRO0823; SLAP-130; FYN-binding protein (FYB-120/130); adhesion and degranulation-promoting adaptor protein; FYB antibody; FYN binding protein (FYB-120/130) antibody; ADAP antibody; PRO0823 antibody; SLAP-130 antibody; FYN-binding protein (FYB-120/130) antibody; adhesion and degranulation-promoting adaptor protein antibody; anti-FYB antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SEEKPDKEPKP
• Sequence Length: 829

• Applicable Applications for anti-FYB antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 2000.; Western Blot: Approx 90kDa band observed in lysates of cell line MOLT4 (calculated MW of 90.6kDa according to NP_001456.3). Recommended concentration: 0.2-0.6ug/ml.
• Immunogen: Peptide with sequence C-SEEKPDKEPKP, from the internal region of the protein sequence according to NP_001456.3; NP_955367.1; NP_001230022.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise all reported isoforms (NP_001456.3; NP_955367.1; NP_001230022.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.2ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 42476118
• NCBI GeneID: 2533
• NCBI Accession #: NP_001456.3; NP_955367.1; NP_001230022.1
• NCBI GenBank Nucleotide #: NM_001465.4
• Molecular Weight: 91,651 Da
• NCBI Official Full Name: FYN-binding protein isoform 1
• NCBI Official Synonym Full Names: FYN binding protein
• NCBI Official Symbol: FYB
• NCBI Official Synonym Symbols: ADAP; PRO0823; SLAP130; SLAP-130
• NCBI Protein Information: FYN-binding protein
• UniProt Protein Name: FYN-binding protein
• Protein Family: FYN-binding protein
• UniProt Gene Name: FYB
• UniProt Synonym Gene Names: SLAP130; ADAP; p120/p130
• UniProt Entry Name: FYB_HUMAN

NCBI Description

The protein encoded by this gene is an adapter for the FYN protein and LCP2 signaling cascades in T-cells. The encoded protein is involved in platelet activation and controls the expression of interleukin-2. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

SLAP-130: an adapter protein for fyn and the SH2-domain-containing leucocyte protein-76 (SLP76) in T cells. T-cell receptor ligation leads to increased tyrosine phosphorylation of SLAP-130. Two alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: actin cytoskeleton; cytosol; nucleus; plasma membrane

Molecular Function: protein binding; protein complex binding; receptor binding

Biological Process: immune response; NLS-bearing substrate import into nucleus; protein amino acid phosphorylation; signal transduction; T cell receptor signaling pathway