Structure Specific Recognition Protein 1 Recombinant Protein | SSRP1 recombinant protein

Recombinant Structure Specific Recognition Protein 1 (SSRP1)

Cat. Num. AAA2031105

• Gene Names: SSRP1; FACT; T160; FACT80
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Structure Specific Recognition Protein 1; Recombinant Structure Specific Recognition Protein 1 (SSRP1); SSRP1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.7 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EFNDV YQEVKGSMND GRLRLSRQGI IFKNSKTGKV DNIQAGELTE GIWRRVALGH GLKLLTKNGH VYKYDGFRES EFEKLSDFFK THYRLELMEK DLCVKGWNWG TVKFGGQLLS FDIGDQPVFE IPLSNVSQCT TGKNEVTLEF HQNDDAEVSL MEVRFYVPPT QEDGVDPVEA FAQNVLSKAD VIQATGDAICIFRELQCLTP RGRYDIRIYP TFLHLHGKTF DYKIPYTTVL RLFLLP
• Sequence Length: 709

• Applicable Applications for SSRP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.2kDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Glu6~Pro246
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13477285
• NCBI GeneID: 6749
• Molecular Weight: 81,075 Da
• NCBI Official Full Name: Structure specific recognition protein 1
• NCBI Official Synonym Full Names: structure specific recognition protein 1
• NCBI Official Symbol: SSRP1
• NCBI Official Synonym Symbols: FACT; T160; FACT80
• NCBI Protein Information: FACT complex subunit SSRP1
• UniProt Protein Name: FACT complex subunit SSRP1
• UniProt Gene Name: SSRP1
• UniProt Synonym Gene Names: FACT80; FACT 80 kDa subunit; FACTp80; hSSRP1
• UniProt Entry Name: SSRP1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

SSRP1: a subunit of the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63.

Protein type: Nucleolus; RNA-binding; Transcription, coactivator/corepressor; Transcription initiation complex

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; cytoplasm; nucleolus; chromosome; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; chromatin remodeling; regulation of transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; DNA replication; DNA repair