Sheep anti-Human Fucosyltransferase 5 Polyclonal Antibody | anti-FUT5 antibody
Fucosyltransferase 5 (alpha (1,3) Fucosyltransferase, FUT5)
Gene Names
FUT5; FUT6; fut356
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Fucosyltransferase 5; Polyclonal Antibody; Fucosyltransferase 5 (alpha (1; 3) Fucosyltransferase; FUT5); Anti -Fucosyltransferase 5 (alpha (1; anti-FUT5 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FUT5 and FUT3 at 43 and 42kD, respectively, with similar sensitivity.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-FUT5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Immunoprecipitation, ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml. The detection limit for rhFUT3 and rhFUT5 is ~0.3ng/ml.
Western Blot: 0.1-0.2ug/ml. The detection limit for rhFUT3 and rhFUT5 is ~1ng/ml under non-reducing and reducing conditions.
Immunoprecipitation: Used to immunoprecipitate rhFUT3/5 from conditioned media of transfected NSO cells.
Dilution: ELISA: 0.5-1ug/ml. The detection limit for rhFUT3 and rhFUT5 is ~0.3ng/ml.
Western Blot: 0.1-0.2ug/ml. The detection limit for rhFUT3 and rhFUT5 is ~1ng/ml under non-reducing and reducing conditions.
Immunoprecipitation: Used to immunoprecipitate rhFUT3/5 from conditioned media of transfected NSO cells.
Immunogen
NS0-derived recombinant human FUT5 lumenal domain Accession # Q11128
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-FUT5 antibody
FUT5 (alpha-1, 3-fucosyltransferase 5; also Fuc-TV) is a 43kD member of the glycosyltransferase 10 family. It is responsible for the terminal step in the synthesis of Le, sialyl-Le, and Le antigens. This is accomplished by the transfer of fucose to preexisting LacNAc or sialyl-LacNAc structures. Human FUT5 is 374 amino acids (aa) in length. It is a type II transmembrane Golgi glycoprotein with a C-terminal lumenal domain (aa35-374). The catalytic domain spans aa77-374, while the acceptor-binding motif includes aa120-126. FUT5 is the product of a single exon gene. Thus, there are no splice variants. FUT5 only occurs in primates; there is no rodent ortholog.
Product Categories/Family for anti-FUT5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fucosyltransferase 5
NCBI Official Symbol
FUT5
NCBI Official Synonym Symbols
FUT6; fut356
NCBI Protein Information
fucosyltransferase 5; alpha-1,3-fucosyltransferase; fucosyltransferase 6 (alpha (1,3) fucosyltransferase)
Protein Family
Similar Products
Product Notes
The FUT5 (Catalog #AAA610250) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Fucosyltransferase 5 (alpha (1,3) Fucosyltransferase, FUT5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fucosyltransferase 5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Immunoprecipitation, ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml. The detection limit for rhFUT3 and rhFUT5 is ~0.3ng/ml. Western Blot: 0.1-0.2ug/ml. The detection limit for rhFUT3 and rhFUT5 is ~1ng/ml under non-reducing and reducing conditions. Immunoprecipitation: Used to immunoprecipitate rhFUT3/5 from conditioned media of transfected NSO cells. Researchers should empirically determine the suitability of the FUT5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fucosyltransferase 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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