Rabbit FUBP1 Polyclonal Antibody | anti-FUBP1 antibody

FUBP1, ID (FUBP1, Far upstream element-binding protein 1, DNA helicase V) (Azide free) (HRP)

Cat. Num. AAA6300294

• Gene Names: FUBP1; FBP; FUBP; hDH V
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: FUBP1; Polyclonal Antibody; ID (FUBP1; Far upstream element-binding protein 1; DNA helicase V) (Azide free) (HRP); DNA helicase V; anti-FUBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FUBP1. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FUBP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 240-268 in the central region of human FUBP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251, rat C6 cell line, mouse brain, rat brain tissue lysates (35ug/lane) usingMBS6011658. This demonstrates MBS6011658 detected the FUBP1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis using MBS6011658 (1:1000) Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: U-251 MG whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 68kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-FUBP1 antibody

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

Product Categories/Family for anti-FUBP1 antibody

Antibodies; Binding Proteins

References

1. Hsiao, H.H., et al. Biochemistry 49(22):4620-4634(2010). 2. Rabenhorst, U., et al. Hepatology 50(4):1121-1129(2009). 3. Malz, M., et al. Hepatology 50(4):1130-1139(2009). 4. Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009). 5. Jang, M., et al. Oncogene 28(12):1529-1536(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 17402900
• NCBI GeneID: 8880
• NCBI Accession #: NP_003893.2
• NCBI GenBank Nucleotide #: NM_003902.4
• UniProt Accession #: Q96AE4; Q12828
• Molecular Weight: 68,605 Da
• NCBI Official Full Name: far upstream element-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: far upstream element (FUSE) binding protein 1
• NCBI Official Symbol: FUBP1
• NCBI Official Synonym Symbols: FBP; FUBP; hDH V
• NCBI Protein Information: far upstream element-binding protein 1; DNA helicase V
• UniProt Protein Name: Far upstream element-binding protein 1
• Protein Family: Far upstream element-binding protein
• UniProt Gene Name: FUBP1
• UniProt Synonym Gene Names: FBP; FUSE-binding protein 1; hDH V
• UniProt Entry Name: FUBP1_HUMAN

NCBI Description

The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

FBP1: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Motility/polarity/chemotaxis; RNA-binding; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity; single-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter