Sorting nexin-1 Recombinant Protein | SNX1 recombinant protein

Recombinant Human Sorting nexin-1

Cat. Num. AAA1398606

• Gene Names: SNX1; VPS5; HsT17379
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Sorting nexin-1; Recombinant Human Sorting nexin-1; SNX1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-522aa; Full Length
• Sequence: MASGGGGCSASERLPPPFPGLEPESEGAAGGSEPEAGDSDTEGEDIFTGAAVVSKHQSPKITTSLLPINNGSKENGIHEEQDQEPQDLFADATVELSLDSTQNNQKKVLAKTLISLPPQEATNSSKPQPTYEELEEEEQEDQFDLTVGITDPEKIGDGMNAYVAYKVTTQTSLPLFRSKQFAVKRRFSDFLGLYEKLSEKHSQNGFIVPPPPEKSLIGMTKVKVGKEDSSSAEFLEKRRAALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVGTQTLSGAGLLKMFNKATDAVSKMTIKMNESDIWFEEKLQEVECEEQRLRKLHAVVETLVNHRKELALNTAQFAKSLAMLGSSEDNTALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRWQDAQATLQKKREAEARLLWANKPDKLQQAKDEILEWESRVTQYERDFERISTVVRKEVIRFEKEKSKDFKNHVIKYLETLLYSQQQLAKYWEAFLPEAKAIS
• Sequence Length: 557

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for SNX1 recombinant protein

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1) and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvent in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking.

Product Categories/Family for SNX1 recombinant protein

Signal Transduction

References

Enhanced degradation of EGF receptors by a sorting nexin, SNX1.Kurten R.C., Cadena D.L., Gill G.N.Science 272:1008-1010(1996)

NCBI and Uniprot Product Information

• NCBI GI #: 339409190
• NCBI GeneID: 6642
• NCBI Accession #: NP_001229862.1
• NCBI GenBank Nucleotide #: NM_001242933.1
• UniProt Accession #: Q13596; O60750; O60751; Q6ZRJ8; A6NM19; A8K6T7; H0Y2M5
• Molecular Weight: 75.1 kDa
• NCBI Official Full Name: sorting nexin-1 isoform d
• NCBI Official Synonym Full Names: sorting nexin 1
• NCBI Official Symbol: SNX1
• NCBI Official Synonym Symbols: VPS5; HsT17379
• NCBI Protein Information: sorting nexin-1
• UniProt Protein Name: Sorting nexin-1
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX1
• UniProt Entry Name: SNX1_HUMAN

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

SNX1: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3- phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Belongs to the sorting nexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytoplasm; cytosol; early endosome membrane; endosome membrane; extrinsic to membrane; Golgi apparatus; intracellular membrane-bound organelle; lamellipodium; membrane; protein complex; retromer complex

Molecular Function: epidermal growth factor receptor binding; identical protein binding; insulin receptor binding; phosphoinositide binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: intracellular protein transport; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of protein catabolic process; receptor internalization; retrograde transport, endosome to Golgi; vesicle organization and biogenesis