Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Doublecortex/DCX Antibody | anti-DCX antibody
Goat anti-Doublecortex/DCX Antibody
Expected from sequence similarity: Human, Rat, Dog, Cat
Expected from sequence similarity: Human, Rat, Dog, Cat
WB: Approx 45kDa band observed in Mouse fetal Brain lysates. Recommended concentration: 0.003-0.01ug/ml. Primary incubation 1 hour at room temperature.
IF: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunohistochemistry (IHC)
((0.003ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((0.003ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
Uniprot Description
Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Research Articles on DCX
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Product Notes
The DCX dcx (Catalog #AAA423716) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Doublecortex/DCX Antibody reacts with Mouse Expected from sequence similarity: Human, Rat, Dog, Cat and may cross-react with other species as described in the data sheet. AAA Biotech's Doublecortex/DCX can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:128000. WB: Approx 45kDa band observed in Mouse fetal Brain lysates. Recommended concentration: 0.003-0.01ug/ml. Primary incubation 1 hour at room temperature. IF: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml. Researchers should empirically determine the suitability of the DCX dcx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Doublecortex/DCX, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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