Chicken TrkA Polyclonal Antibody | anti-TrkA antibody

Chicken Polyclonal Anti-TrkA antibody.

Cat. Num. AAA5400171

• Gene Names: NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: TrkA; Polyclonal Antibody; Chicken Polyclonal Anti-TrkA antibody.; CIPA; gp140trk; High affinity nerve growth factor receptor; MTC; Neurotrophic tyrosine kinase receptor type 1; Ntrk1; Oncogene TRK; p140-TrkA; Slow nerve growth; TRK; Trk-A; TRK1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor A antibody; anti-TrkA antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified Immunoglobulins
• Concentration: 0.60 ug/ul IgY in antibody stabilization buffer (varies by lot)
• Sequence Length: 796

• Applicable Applications for anti-TrkA antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 1:10,000; Immunoprecipitation: 1:200; Western Blot: 1:500; Immunohistochemistry: 1:200; DOT Blot: 1:10,000; ; Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.
• Immunogen: Synthetic peptide taken within amino acid region 360-410 on human TrkA protein.
• Immunoglobulin: IgY
• Expression: Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
• Molecular Function: Developmental Protein; Kinase; Receptor; Transferase; Tyrosine-protein kinase
• Structure: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 protein kinase domain.
• Subcellular Location: Cell membrane; Single-pass type I membrane protein; Early & Late endosome membrane
• Related Product: FITC-Conjugated: MBS5400173; Antigenic Blocking Peptide:MBS544618
• Preparation and Storage: -20 degree C for long term storage

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Related Product Information for anti-TrkA antibody

Receptor tyrosine kinases (RTK)s are high-affinity cell surface receptors for many polypeptide rowth factors, cytokines, and hormones (1). TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC (2). Tyrosine Receptor Kinase A (TrkA) is a receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic nervous neurons. TrkA is a high affinity receptor for NGF, its primary ligand, but also binds and can be activated via NTF3/neutrophin-3. NGF is important in both local and nuclear actions, regulating growth ones, motility, and expression of genes encoding biosynthesis of enzymes for neurotransmitters. In absence of ligand and activation, may promote cell death. TrkA belongs to the protein kinase superfamily, specifically the tyrosine protein kinase family.

The TrkA-selective antibodies were generated using unique peptides from an internal sequence corresponding to amino acid region 360-410 of the TrkA protein. The TrkA-selective antibodies were affinity purified against immobilized antigen based affinity chromatography and are represented a~ pure IgG fractions stabilized in antibody stabilization buffer. Synthetic blocking peptides and western blot positive controls are available. Antibodies can be conjugated to fluorescent probes or secondary enzymes upon request at extra charge.

Product Categories/Family for anti-TrkA antibody

Primary Antibodies; Protein Kinases; TrkA Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 3869113
• NCBI GeneID: 4914
• UniProt Accession #: P04629; P08119; Q15655; Q15656; Q5D056; Q5VZS2; Q7Z5C3; Q9UIU7; B2R6T5; B7ZM34
• Molecular Weight: 77,145 Da
• NCBI Official Full Name: TRKA
• NCBI Official Synonym Full Names: neurotrophic receptor tyrosine kinase 1
• NCBI Official Symbol: NTRK1
• NCBI Official Synonym Symbols: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• NCBI Protein Information: high affinity nerve growth factor receptor
• UniProt Protein Name: High affinity nerve growth factor receptor
• Protein Family: Trk system potassium uptake protein
• UniProt Gene Name: NTRK1
• UniProt Synonym Gene Names: MTC; TRK; TRKA; Trk-A
• UniProt Entry Name: NTRK1_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Oncoprotein; Kinase, protein; Membrane protein, integral; TK group; Trk family

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: axon; cell soma; cell surface; cytoplasmic vesicle; dendrite; early endosome; early endosome membrane; endosome; integral to plasma membrane; late endosome; late endosome membrane; plasma membrane; protein complex; receptor complex

Molecular Function: ATP binding; GPI-linked ephrin receptor activity; kinase binding; nerve growth factor binding; nerve growth factor receptor activity; neurotrophin binding; neurotrophin p75 receptor binding; protein binding; protein homodimerization activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: activation of MAPKK activity; activation of NF-kappaB transcription factor; aging; axon guidance; B cell differentiation; circadian rhythm; detection of mechanical stimulus involved in sensory perception of pain; detection of temperature stimulus involved in sensory perception of pain; ephrin receptor signaling pathway; learning and/or memory; mechanoreceptor differentiation; microtubule-based movement; negative regulation of cell proliferation; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; olfactory nerve development; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; positive regulation of angiogenesis; positive regulation of GTPase activity; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; response to activity; response to axon injury; response to drug; response to electrical stimulus; response to ethanol; response to hydrostatic pressure; response to nutrient levels; response to radiation; Sertoli cell development; sympathetic nervous system development

Disease: Insensitivity To Pain, Congenital, With Anhidrosis; Thyroid Carcinoma, Familial Medullary