Rabbit anti-Human FTH1 Polyclonal Antibody | anti-FTH1 antibody

FTH1, CT (Ferritin Heavy Chain, Ferritin H Subunit, Cell Proliferation-inducing Gene 15 Protein, PIG15, OK/SW-cl.84, FTHL6, FTH) (MaxLight 550)

Cat. Num. AAA6475871

• Gene Names: FTH1; FHC; FTH; PLIF; FTHL6; PIG15
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: FTH1; Polyclonal Antibody; CT (Ferritin Heavy Chain; Ferritin H Subunit; Cell Proliferation-inducing Gene 15 Protein; PIG15; OK/SW-cl.84; FTHL6; FTH) (MaxLight 550); anti-FTH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FTH1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-FTH1 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: FC/FACS: 1:10-1:50.; WB: 1:100-1:500; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa154-183 in the C-terminal region of human FTH1, UniProt Accession #NP_002023.2, P02794
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of A2058 cell line lysates (35ug/lane) usingMBS629951. FTH1 (arrow) was detected using theMBS629951.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS629951, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells (bottom histogram) using MBS629951 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FTH1 antibody

Antibodies; Serum Proteins

References

1. Almeida, R.S., et.al., PLoS Pathog. 4(11), E1000217 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 56682959
• NCBI GeneID: 2495
• NCBI Accession #: NP_002023.2
• NCBI GenBank Nucleotide #: NM_002032.2
• UniProt Accession #: P02794; Q3KRA8; Q3SWW1; B3KNR5
• Molecular Weight: 21,226 Da
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; PLIF; FTHL6; PIG15
• NCBI Protein Information: ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
• UniProt Protein Name: Ferritin heavy chain
• Protein Family: Iron transporter
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6; Ferritin H subunit
• UniProt Entry Name: FRIH_HUMAN

NCBI Description

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Oxidoreductase; EC 1.16.3.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; cytosol; nucleus

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: receptor-mediated endocytosis; negative regulation of cell proliferation; cellular iron ion homeostasis; negative regulation of fibroblast proliferation; immune response; post-Golgi vesicle-mediated transport; iron ion transport; transmembrane transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5