Mouse BIRC5 Monoclonal Antibody | anti-BIRC5 antibody

BIRC5 antibody

Cat. Num. AAA533146

• Gene Names: BIRC5; API4; EPR-1
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: BIRC5 antibody was purified by affinity chromatography.
• Synonyms: BIRC5; Monoclonal Antibody; BIRC5 antibody; Monoclonal BIRC5; Anti-BIRC5; Survivin; Baculoviral Inhibitor of Apoptosis repeat-Containing 5; BIRC 5; BIRC-5; anti-BIRC5 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2D3
• Purity/Purification: BIRC5 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 142

• Applicable Applications for anti-BIRC5 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; WB: 1:2000
• Biological Significance: BIRC5 is a human gene encoding a structurally unique IAP apoptosis inhibitor. Present during fetal development, survivin is undetectable in terminally differentiated adult tissues. However, survivin becomes prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast, in vivo.
• Cross-Reactivity: Human
• Immunogen: BIRC5 antibody was raised in mouse using a full length recombinant protein of human BIRC5 (NP_001159) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BIRC5 protein (Right) or empty vector (Left) detected with BIRC5 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant BIRC5 protein using BIRC5 antibody)

Related Product Information for anti-BIRC5 antibody

Mouse monoclonal BIRC5 antibody

Product Categories/Family for anti-BIRC5 antibody

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 59859878
• NCBI GeneID: 332
• NCBI Accession #: NP_001159
• NCBI GenBank Nucleotide #: NM_001168.2
• UniProt Accession #: O15392; Q2I3N8; Q4VGX0; Q53F61; Q5MGC6; Q6FHL2; Q75SP2; Q9P2W8; A2SUH6; B2R4R1
• Molecular Weight: 8,490 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 5 isoform 1
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 5
• NCBI Official Symbol: BIRC5
• NCBI Official Synonym Symbols: API4; EPR-1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 5
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 5
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC5
• UniProt Synonym Gene Names: API4; IAP4
• UniProt Entry Name: BIRC5_HUMAN

NCBI Description

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: centriole; nuclear chromosome; microtubule; spindle microtubule; cytoplasmic microtubule; cytoplasm; spindle; midbody; cytosol; nucleus; interphase microtubule organizing center; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein homodimerization activity; zinc ion binding; metal ion binding; microtubule binding; caspase inhibitor activity; ubiquitin-protein ligase activity; protein binding; enzyme binding; protein heterodimerization activity; chaperone binding; cofactor binding; cysteine protease inhibitor activity; Ran GTPase binding; cobalt ion binding

Biological Process: mitosis; transcription, DNA-dependent; spindle checkpoint; establishment of chromosome localization; protein ubiquitination; regulation of signal transduction; cytokinesis; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; positive regulation of exit from mitosis; negative regulation of apoptosis