Western Blot (WB)
( Western blot analysis of FRS2 phosphorylation expression in Hela whole cell lysates, The lane on the right is treated with the antigen-specific peptide. )
Rabbit anti-Human, Mouse FRS2 Polyclonal Antibody | anti-FRS2 antibody
Phospho-FRS2 (Tyr436) Antibody
Gene Names
FRS2; SNT; SNT1; FRS1A; FRS2A; SNT-1; FRS2alpha
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
FRS2; Polyclonal Antibody; Phospho-FRS2 (Tyr436) Antibody; FGFR signaling adaptor SNT; FGFR signalling adaptor; FGFR substrate 2; FGFR-signaling adaptor SNT; Fibroblast growth factor receptor substrate 2; FRS 2; FRS2_HUMAN; FRS2A; FRS2alpha; SNT 1; SNT; SNT-1; SNT1; Suc 1 Associated Neurotrophic Factor Target; Suc1 associated neurotrophic factor target 1; Suc1-associated neurotrophic factor target 1; anti-FRS2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-FRS2 (Tyr436) antibody detects endogenous levels of FRS2 only when phosphorylated at Tyrosine 436
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
508
Applicable Applications for anti-FRS2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human FRS2 around the phosphorylation site of Tyrosine 436
Subcellular Location
Endomembrane System. Cytoplasmic, Membrane-Bound.
Tissue Specificity
Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Conjugation
Unconjugated
Epitope
Phospho Tyr436
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
( Western blot analysis of FRS2 phosphorylation expression in Hela whole cell lysates, The lane on the right is treated with the antigen-specific peptide. )
Western Blot (WB)
( Western blot analysis of FRS2 phosphorylation expression in Hela whole cell lysates, The lane on the right is treated with the antigen-specific peptide. )
Immunohistochemistry (IHC)
(Phospho-FRS2 (Tyr436) Antibody for IHC in human brain tissue )
Immunohistochemistry (IHC)
(Phospho-FRS2 (Tyr436) Antibody for IHC in human brain tissue )
Western Blot (WB)
(Western blot analysis of FRS2 phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of FRS2 phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-FRS2 antibody
Description: FRS2 is an adaptor protein involved in fibroblast growth factor receptor (FGFR) signaling. Plays an important role in linking FGFR and nerve growth factor receptors with Ras/MAPK signaling pathways.
Function: Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
Subunit Structure: Part of a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Part of a complex containing GRB2 and CBL. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds RET (By similarity). Binds ALK, FGFR1, CKS2, MAPK1/ERK2, MAPK3/ERK1 and SRC. The tyrosine-phosphorylated protein binds the SH2 domains of GRB2 and PTPN11. Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated upon ligand-binding).
Post-translational Modifications: Phosphorylated by ULK2 in vitro (By similarity). Phosphorylated on tyrosine residues upon stimulation by NGF or FGF2. Phosphorylated on tyrosine residues by activated ALK and FGFR1. Phosphorylated on tyrosine residues upon activation of FGFR2 and FGFR3. Phosphorylated on threonine residues by MAP kinases; this inhibits tyrosine phosphorylation, and thereby down-regulates FRS2-mediated activation of MAP kinases. Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination (By similarity).
Function: Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
Subunit Structure: Part of a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Part of a complex containing GRB2 and CBL. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds RET (By similarity). Binds ALK, FGFR1, CKS2, MAPK1/ERK2, MAPK3/ERK1 and SRC. The tyrosine-phosphorylated protein binds the SH2 domains of GRB2 and PTPN11. Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated upon ligand-binding).
Post-translational Modifications: Phosphorylated by ULK2 in vitro (By similarity). Phosphorylated on tyrosine residues upon stimulation by NGF or FGF2. Phosphorylated on tyrosine residues by activated ALK and FGFR1. Phosphorylated on tyrosine residues upon activation of FGFR2 and FGFR3. Phosphorylated on threonine residues by MAP kinases; this inhibits tyrosine phosphorylation, and thereby down-regulates FRS2-mediated activation of MAP kinases. Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 65 kDa
Predicted: 58 kDa
Predicted: 58 kDa
NCBI Official Full Name
fibroblast growth factor receptor substrate 2
NCBI Official Synonym Full Names
fibroblast growth factor receptor substrate 2
NCBI Official Symbol
FRS2
NCBI Official Synonym Symbols
SNT; SNT1; FRS1A; FRS2A; SNT-1; FRS2alpha
NCBI Protein Information
fibroblast growth factor receptor substrate 2
UniProt Protein Name
Fibroblast growth factor receptor substrate 2
Protein Family
UniProt Gene Name
FRS2
UniProt Synonym Gene Names
FGFR substrate 2; SNT-1
Uniprot Description
Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
Research Articles on FRS2
Similar Products
Product Notes
The FRS2 frs2 (Catalog #AAA9601235) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-FRS2 (Tyr436) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FRS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the FRS2 frs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FRS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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