Rabbit Frizzled-8 Polyclonal Antibody | anti-K528 antibody

Frizzled-8 (K528) Polyclonal Antibody

Cat. Num. AAA3002977

• Gene Names: FZD8; FZ-8; hFZ8
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Frizzled-8; Polyclonal Antibody; Frizzled-8 (K528) Polyclonal Antibody; Fz-8; hFz8; FZD8; FZ-8; Hfz8; Frizzled 8; Frizzled8; anti-K528 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Frizzled-8 (K528) polyclonal antibody detects endogenous levels of Frizzled-8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 694

• Applicable Applications for anti-K528 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 490-537 of Human Frizzled-8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Frizzled-8 (K528) polyclonal antibody at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug) Lane4:COS-7 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Frizzled-8 (K528) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Frizzled-8 (K528) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-K528 antibody

The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins (FRPs), have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy-terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. The cysteine-rich domain of frizzled-8 blocks endogenous Wnts and the effects of Wnt-1 and Wnt-5 on proliferation. The mouse frizzled-8 gene, which encodes a Wnt receptor, is a potent cancer-associated activator of the beta-catenin-TCF pathway. The frizzled-8 gene contains no introns. Frizzled-8 mRNA has been detected in fetal brain and kidney, and also in adult pancreas, skeletal muscle, kidney and heart. Frizzled is highly expressed in HeLa S3 (cervical uterus cancer) cells and A549 lung cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 13994190
• NCBI GeneID: 8325
• NCBI Accession #: NP_114072.1
• NCBI GenBank Nucleotide #: NP_114072.1
• UniProt Accession #: Q9H461
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: frizzled-8
• NCBI Official Synonym Full Names: frizzled class receptor 8
• NCBI Official Symbol: FZD8
• NCBI Official Synonym Symbols: FZ-8; hFZ8
• NCBI Protein Information: frizzled-8
• UniProt Protein Name: Frizzled-8
• Protein Family: Frizzled
• UniProt Gene Name: FZD8
• UniProt Synonym Gene Names: Fz-8; hFz8
• UniProt Entry Name: FZD8_HUMAN

NCBI Description

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD8: Receptor for Wnt proteins. Component of the Wnt-Fzd- LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome- sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: Golgi apparatus; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; ubiquitin protein ligase binding; PDZ domain binding; receptor binding

Biological Process: neuron differentiation; positive regulation of JNK activity; G-protein coupled receptor protein signaling pathway; T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin