Rabbit FZD8 / Frizzled 8 Polyclonal Antibody | anti-FZD8 antibody

Rabbit Polyclonal to Human FZD8 / Frizzled 8

Cat. Num. AAA244812

• Gene Names: FZD8; FZ-8; hFZ8
• Reactivity: Bovine, Xenopus, Horse, Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: FZD8 / Frizzled 8; Polyclonal Antibody; Rabbit Polyclonal to Human FZD8 / Frizzled 8; Anti-FZD8 / Frizzled 8 Antibody (N-Terminus) IHC-plus; FZD8; Frizzled family receptor 8; Frizzled homolog 8; FZ-8; Fz8; Frizzled 8; Frizzled-8; HFZ8; Human FZD8; anti-FZD8 antibody

• Host: Rabbit
• Reactivity: Bovine, Xenopus, Horse, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human FZD8 / Frizzled 8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 694

• Applicable Applications for anti-FZD8 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (7 - 17 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 12 amino acid peptide from N-terminal extracellular domain of human FZD8 / Frizzled 8.
• Immunogen Type: Synthetic peptide
• Immunogen: FZD8 / Frizzled 8 antibody was raised against synthetic 12 amino acid peptide from N-terminal extracellular domain of human FZD8 / Frizzled 8. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Panda, Bovine, Horse, Opossum, Xenopus (100%); Lizard, Seabass, Pufferfish (83%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-FZD8 / Frizzled 8 antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-FZD8 / Frizzled 8 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-FZD8 / Frizzled 8 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-FZD8 / Frizzled 8 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-FZD8 / Frizzled 8 antibody IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-FZD8 antibody

FZD8 is a Frizzled Receptor that mediates Wnt signaling and is involved in determining vertebrate axis and tissue polarity. FZD8 is expressed during embryonic development in forming and differentiating somites in mice.

Product Categories/Family for anti-FZD8 antibody

Family: GPCR; Subfamily: Frizzled

NCBI and Uniprot Product Information

• NCBI GI #: 13994190
• NCBI GeneID: 8325
• NCBI Accession #: NP_114072.1
• NCBI GenBank Nucleotide #: NM_031866.2
• UniProt Accession #: Q9H461
• Molecular Weight: 73,300 Da
• NCBI Official Full Name: frizzled-8
• NCBI Official Synonym Full Names: frizzled class receptor 8
• NCBI Official Symbol: FZD8
• NCBI Official Synonym Symbols: FZ-8; hFZ8
• NCBI Protein Information: frizzled-8; frizzled homolog 8; frizzled family receptor 8; frizzled 8, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-8
• Protein Family: Frizzled
• UniProt Gene Name: FZD8
• UniProt Synonym Gene Names: Fz-8; hFz8
• UniProt Entry Name: FZD8_HUMAN

NCBI Description

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD8: Receptor for Wnt proteins. Component of the Wnt-Fzd- LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome- sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: Golgi apparatus; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; ubiquitin protein ligase binding; receptor binding; PDZ domain binding

Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of JNK activity; T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin