Rabbit FOXP3 Polyclonal Antibody | anti-FOXP3 antibody
FOXP3 Antibody FITC-Conjugated
Reactivity
Bovine, Cat, Human, Monkey, Mouse, Pig, RatPredicted: Pig, Cynomolgus monkey, Rhesus monkey, Deer
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
FOXP3; Polyclonal Antibody; FOXP3 Antibody FITC-Conjugated; AIID; DIETER; Forkhead box P3; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; IPEX; JM2; MGC141961; MGC141963; PIDX; SCURFIN; XPID antibody; anti-FOXP3 antibody
Host
Rabbit
Reactivity
Bovine, Cat, Human, Monkey, Mouse, Pig, Rat
Predicted: Pig, Cynomolgus monkey, Rhesus monkey, Deer
Predicted: Pig, Cynomolgus monkey, Rhesus monkey, Deer
Clonality
Polyclonal
Form/Format
FITC-Conjugated
Concentration
0.7 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
150
Applicable Applications for anti-FOXP3 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/ Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ELISA: 1:15,000-1:20,000
Immunocytochemistry: 1:100
Immunofluorescence: 1:100
Immunohistochemistry: 1:100
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunocytochemistry: 1:100
Immunofluorescence: 1:100
Immunohistochemistry: 1:100
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunogen
Synthetic peptide corresponding to amino acids 229-250 of Mouse Forkhead Box Protein 3 (winged helix transcription factor 3 protein). The peptide was post synthetically modified to achieve desired antigenicity before coupling to a carrier protein.
Sequence: AQCLLQREVVQSLEQQLELEKE
Sequence: AQCLLQREVVQSLEQQLELEKE
Immunoglobulin
IgG
Expression
Down-regulated in regulatory T-cells (Treg) during inflammation.
Molecular Function
Core promoter binding; Histone Acetyltransferase binding; Histone deacetylase binding; NFAT protein binding; NF-kappaB binding; Activator; Repressor
Structure
Homodimer. Interacts with IKZF3. Isoform 1 (via LXXLL motif), but not isoform 2, interacts with isoform 4 of RORA (via AF-2 motif). Interacts with STUB1, HSPA8 and HSPA1A/B. Interacts with PPP1CA, PPP1CB and PPP1CG. Interacts with KAT5 and HDAC7. Interacts with HDAC9 in the absence of T-cell stimulation. Interacts with USP7. Interacts with isoform 2 of ZFP90 and can form a complex with TRIM28 in the presence of isoform 2 of ZFP90. Interacts with RUNX1. Interacts with RORC. Interacts with RELA and NFATC2. Interacts with RUNX2, RUNX3 and IKZF4.
Subcellular Location
Nucleus; Cytoplasm
Preparation and Storage
-20 degree C for long term storage
Related Product Information for anti-FOXP3 antibody
FITC-Conjugated Forkhead box protein 3 Antibody
Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,785 Da
NCBI Official Full Name
UPF0686 protein C11orf1
NCBI Official Synonym Full Names
chromosome 11 open reading frame 1
NCBI Official Symbol
C11orf1
NCBI Protein Information
UPF0686 protein C11orf1
UniProt Protein Name
UPF0686 protein C11orf1
Protein Family
UniProt Gene Name
C11orf1
UniProt Entry Name
CK001_HUMAN
Uniprot Description
C11orf1: Belongs to the UPF0686 family.
Chromosomal Location of Human Ortholog: 11q23.1
Cellular Component: nucleoplasm; nucleus
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Product Notes
The FOXP3 c11orf1 (Catalog #AAA541630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FOXP3 Antibody FITC-Conjugated reacts with Bovine, Cat, Human, Monkey, Mouse, Pig, Rat Predicted: Pig, Cynomolgus monkey, Rhesus monkey, Deer and may cross-react with other species as described in the data sheet. AAA Biotech's FOXP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/ Paraffin, Immunoprecipitation (IP), Western Blot (WB). ELISA: 1:15,000-1:20,000 Immunocytochemistry: 1:100 Immunofluorescence: 1:100 Immunohistochemistry: 1:100 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the FOXP3 c11orf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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