Rabbit FOXP1 Polyclonal Antibody | anti-FOXP1 antibody

FOXP1 Polyclonal Antibody

Cat. Num. AAA3012718

• Gene Names: FOXP1; MFH; QRF1; 12CC4; hFKH1B; HSPC215
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: FOXP1; Polyclonal Antibody; FOXP1 Polyclonal Antibody; MFH; 12CC4; FLJ23741; Fork head related protein like B; Forkhead box P1; Forkhead box protein P1; FOX P1; FOXP 1; foxp1; Glutamine rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF 1; QRF1; anti-FOXP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FOXP1 polyclonal antibody detects endogenous levels of FOXP1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 677

• Applicable Applications for anti-FOXP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000-1:5,000; FC/FACS: 1:50-1:100; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of FOXP1 on different lysates using anti-FOXP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: MCF-7)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-FOXP1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-FOXP1 antibody

The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. The gene encoding human FOXP1 maps to chromosome 3p14.1. The gene encoding human FOXP2 maps to chromosome 7q31. The gene encoding FOXP3, a third member of this family, maps to chromosome Xp11.23-Xq13.3. Mutations in this gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein, also known as scurfin, is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T-cell activation.

NCBI and Uniprot Product Information

• NCBI GI #: 348605235
• NCBI GeneID: 27086
• NCBI Accession #: NP_001231745.1
• NCBI GenBank Nucleotide #: NP_001231745.1
• UniProt Accession #: Q9H334; P58462; Q498D1
• Molecular Weight: 77/75kDa
• NCBI Official Full Name: forkhead box protein P1 isoform a
• NCBI Official Synonym Full Names: forkhead box P1
• NCBI Official Symbol: FOXP1
• NCBI Official Synonym Symbols: MFH; QRF1; 12CC4; hFKH1B; HSPC215
• NCBI Protein Information: forkhead box protein P1
• UniProt Protein Name: Forkhead box protein P1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP1
• UniProt Entry Name: FOXP1_HUMAN

NCBI Description

This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP1: Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development. Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; androgen receptor binding; sequence-specific DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of smooth muscle cell proliferation; response to lipopolysaccharide; osteoclast differentiation; endothelial cell activation; regulation of transcription from RNA polymerase II promoter; macrophage activation; regulation of monocyte differentiation; monocyte activation; regulation of inflammatory response; regulation of interleukin-1 beta secretion; regulation of tumor necrosis factor production; negative regulation of transcription, DNA-dependent; negative regulation of B cell apoptosis

Disease: Mental Retardation With Language Impairment And With Or Without Autistic Features