Mouse Growth-regulated nocogenea/melanoma growth stimulating ELISA Kit | GROa/MGSA elisa kit

Mouse Growth-regulated nocogenea/melanoma growth stimulating ELISA Kit

Cat. Num. AAA720528

• Gene Names: CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• Reactivity: Mouse
• Synonyms: Growth-regulated nocogenea/melanoma growth stimulating; Mouse Growth-regulated nocogenea/melanoma growth stimulating ELISA Kit; GROa/MGSA elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GRN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GROa/MGSA elisa kit

Principle of the assay: GRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRN antibody and an GRN-HRP conjugate. The assay sample and buffer are incubated together with GRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRN concentration since GRN from samples and GRN-HRP conjugate compete for the anti-GRN antibody binding site. Since the number of sites is limited, as more sites are occupied by GRN from the sample, fewer sites are left to bind GRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRN concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504153
• NCBI GeneID: 2919
• NCBI Accession #: NP_001502.1
• NCBI GenBank Nucleotide #: NM_001511.3
• Molecular Weight: 11,301 Da
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• NCBI Official Symbol: CXCL1
• NCBI Official Synonym Symbols: FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• NCBI Protein Information: growth-regulated alpha protein; MGSA alpha; GRO-alpha(1-73); C-X-C motif chemokine 1; fibroblast secretory protein; neutrophil-activating protein 3; melanoma growth stimulatory activity alpha; GRO1 oncogene (melanoma growth-stimulating activity); GRO1 oncogene (melanoma growth stimulating activity, alpha)
• UniProt Protein Name: Growth-regulated alpha protein
• UniProt Gene Name: CXCL1
• UniProt Synonym Gene Names: GRO; GRO1; GROA; MGSA; SCYB1; MGSA; NAP-3
• UniProt Entry Name: GROA_HUMAN

NCBI Description

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; enzyme activator activity; receptor binding

Biological Process: positive regulation of catalytic activity; G-protein coupled receptor protein signaling pathway; cell proliferation; nervous system development; negative regulation of cell proliferation; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; chemotaxis; signal transduction; actin cytoskeleton organization and biogenesis; inflammatory response