Rabbit anti-Human FOXN1 Polyclonal Antibody | anti-FOXN1 antibody

FOXN1, ID (FOXN1, RONU, WHN, Forkhead box protein N1, Winged-helix transcription factor nude) (FITC)

Cat. Num. AAA6299886

• Gene Names: FOXN1; WHN; RONU; FKHL20
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FOXN1; Polyclonal Antibody; ID (FOXN1; RONU; WHN; Forkhead box protein N1; Winged-helix transcription factor nude) (FITC); Winged-helix transcription factor nude; anti-FOXN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FOXN1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FOXN1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 350-378 in the central region of human FOXN1, UniProt Accession #O15353. Species sequence homology: mouse
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6009073 in A549, NCI-H460 cell line lysates (35ug/lane). This demonstrates the FOXN1 antibody detected the FOXN1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of A549 cells (right histogram) using MBS6009073 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FOXN1 antibody

Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq].

Product Categories/Family for anti-FOXN1 antibody

Antibodies; Transcription Factors

References

1. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 2. Mandinova, A., et al. J. Clin. Invest. 119(10):3127-3137(2009). 3. Pignata, C., et al. Adv. Exp. Med. Biol. 665, 195-206 (2009). 4. Weiner, L., et al. Cell 130(5):932-942(2007). 5. Nonaka, D., et al. Am. J. Surg. Pathol. 31(7):1038-1044(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 18201913
• NCBI GeneID: 8456
• NCBI Accession #: NP_003584.2
• NCBI GenBank Nucleotide #: NM_003593.2
• UniProt Accession #: O15353; O15352; B2R9Q7
• Molecular Weight: 68,925 Da
• NCBI Official Full Name: forkhead box protein N1
• NCBI Official Synonym Full Names: forkhead box N1
• NCBI Official Symbol: FOXN1
• NCBI Official Synonym Symbols: WHN; RONU; FKHL20
• NCBI Protein Information: forkhead box protein N1; Rowett nude; winged helix nude; winged-helix nude; winged-helix transcription factor nude
• UniProt Protein Name: Forkhead box protein N1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXN1
• UniProt Synonym Gene Names: RONU; WHN
• UniProt Entry Name: FOXN1_HUMAN

NCBI Description

Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXN1: Transcriptional regulator involved in development. Defects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND). A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; keratinocyte differentiation; organ morphogenesis; epidermis development; epithelial cell proliferation; hair follicle development; thymus development; regulation of T cell differentiation in the thymus; defense response; lymphocyte homeostasis

Disease: T-cell Immunodeficiency, Congenital Alopecia, And Nail Dystrophy