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Flow Cytometry (FC/FACS)
Rabbit FOXK1/MNF Polyclonal Antibody | anti-FOXK1 antibody
Anti-FOXK1/MNF Antibody
Gene Names
FOXK1; FOXK1L
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
FOXK1/MNF; Polyclonal Antibody; Anti-FOXK1/MNF Antibody; Forkhead box protein F1; Forkhead-related activator 1; FREAC-1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FOXF1; FKHL5; FREAC1; anti-FOXK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
733
Applicable Applications for anti-FOXK1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.1-0.25ug/ml (Human, Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human FOXK1/MNF recombinant protein (Position: Q175-E671).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunofluorescence (IF)
Immunofluorescence (IF)
Immunofluorescence (IF)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Western Blot (WB)
(Figure 1. Western blot analysis of FOXK1 using anti-FOXK1 antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of FOXK1 using anti-FOXK1 antibody.)
Related Product Information for anti-FOXK1 antibody
Description: Rabbit IgG polyclonal antibody for FOXK1/MNF detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.
Background: Forkhead box protein K1 is a transcription factor of the forkhead box family that in humans is encoded by the FOXK1 gene. It is mapped to 7p22.1. FOXK1 is one of the transcription factors managing the passage from the normal cellular respiration (complete glucose oxidation) to generating ATP and intermediaries for many other biochemical pathways. FOXK1 and its closely relate sibling FOXK2 induce aerobic glycolysis by upregulating the enzymatic machinery required for this (for example, hexokinase-2, phosphofructokinase, pyruvate kinase, and lactate dehydrogenase), while at the same time suppressing further oxidation of pyruvate in the mitochondria by increasing the activity of pyruvate dehydrogenase kinases 1 and 4.
Background: Forkhead box protein K1 is a transcription factor of the forkhead box family that in humans is encoded by the FOXK1 gene. It is mapped to 7p22.1. FOXK1 is one of the transcription factors managing the passage from the normal cellular respiration (complete glucose oxidation) to generating ATP and intermediaries for many other biochemical pathways. FOXK1 and its closely relate sibling FOXK2 induce aerobic glycolysis by upregulating the enzymatic machinery required for this (for example, hexokinase-2, phosphofructokinase, pyruvate kinase, and lactate dehydrogenase), while at the same time suppressing further oxidation of pyruvate in the mitochondria by increasing the activity of pyruvate dehydrogenase kinases 1 and 4.
References
1. awke, T. J., Jiang, N., Garry, D. J. Absence of p21(CIP) rescues myogenic progenitor cell proliferative and regenerative capacity in Foxk1 null mice. J. Biol. Chem. 278: 4015-4020, 2003.
2. Huang, J. T.-J., Lee, V. Identification and characterization of a novel human FOXK1 gene in silico. Int. J. Oncol. 25: 751-757, 2004.
3. Katoh, M., Katoh, M. Identification and characterization of human FOXK1 gene in silico. Int. J. Molec. Med. 14: 127-132, 2004.
2. Huang, J. T.-J., Lee, V. Identification and characterization of a novel human FOXK1 gene in silico. Int. J. Oncol. 25: 751-757, 2004.
3. Katoh, M., Katoh, M. Identification and characterization of human FOXK1 gene in silico. Int. J. Molec. Med. 14: 127-132, 2004.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
forkhead box protein K1
NCBI Official Synonym Full Names
forkhead box K1
NCBI Official Symbol
FOXK1
NCBI Official Synonym Symbols
FOXK1L
NCBI Protein Information
forkhead box protein K1
UniProt Protein Name
Forkhead box protein K1
Protein Family
UniProt Gene Name
FOXK1
UniProt Synonym Gene Names
MNF; MNF
UniProt Entry Name
FOXK1_HUMAN
Uniprot Description
FOXK1: Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 7p22.1
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: protein binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; muscle development; positive regulation of transcription, DNA-dependent; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; cell differentiation
Research Articles on FOXK1
Similar Products
Product Notes
The FOXK1 foxk1 (Catalog #AAA1753148) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FOXK1/MNF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FOXK1/MNF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.1-0.25ug/ml (Human, Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat) ICC: 2ug/ml (Human) IF: 2ug/ml (Human) FC/FACS: 1-3ug/1x106 cells (Human) Direct ELISA: 0.1-0.5ug/ml (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the FOXK1 foxk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXK1/MNF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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