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Western Blot (WB) (Western Blot analysis of FNTB on K562 cells lysates using anti-FNTB antibody at 1/1,000 dilution.)

Rabbit anti-Human, Rat FNTB Polyclonal Antibody | anti-FNTB antibody

FNTB Polyclonal Antibody

Gene Names
FNTB; FPTB
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
ProA affinity purified
Synonyms
FNTB; Polyclonal Antibody; FNTB Polyclonal Antibody; FTase-beta; CAAX farnesyltransferase subunit beta; EC 2.5.1.58; EC=2.5.1.58; Farnesyltransferase; farnesyltransferase CAAX box beta1; farnesyltransferase; CAAX box; beta; FPTB; FTase beta; MGC105303; Protein farnesyltransferase subunit beta; RAS proteins prenyltransferase beta; Ras proteins prenyltransferase subunit beta; anti-FNTB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
FNTB polyclonal antibody detects endogenous levels of FNTB protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
437
Applicable Applications for anti-FNTB antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of FNTB on K562 cells lysates using anti-FNTB antibody at 1/1,000 dilution.)

Western Blot (WB) (Western Blot analysis of FNTB on K562 cells lysates using anti-FNTB antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining FNTB in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining FNTB in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-FNTB antibody
Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical alpha and beta subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an a-b heterodimer. The beta subunit, which is known as FTbeta, CAAX farnesyltransferase subunit beta, or Ras proteins prenyltransferase subunit beta, is a 437 amino acid protein that contains five PFTB repeats and binds the peptide substrate. The alpha subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43kDa
NCBI Official Full Name
protein farnesyltransferase subunit beta
NCBI Official Synonym Full Names
farnesyltransferase, CAAX box, beta
NCBI Official Symbol
FNTB
NCBI Official Synonym Symbols
FPTB
NCBI Protein Information
protein farnesyltransferase subunit beta
UniProt Protein Name
Protein farnesyltransferase subunit beta
UniProt Gene Name
FNTB
UniProt Synonym Gene Names
FTase-beta
UniProt Entry Name
FNTB_HUMAN

Uniprot Description

FNTB: a subunit of farnesyltransferase that is responsible for peptide-binding. Farnesyltransferase catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins.

Protein type: Transferase; EC 2.5.1.58

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: microtubule associated complex; cytosol

Molecular Function: protein binding; farnesyltranstransferase activity; isoprenoid binding; zinc ion binding; drug binding; peptide binding; protein farnesyltransferase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; protein farnesylation; response to inorganic substance; regulation of rhodopsin mediated signaling; wound healing; response to cytokine stimulus; positive regulation of cell cycle; response to organic cyclic substance; positive regulation of nitric-oxide synthase biosynthetic process

Research Articles on FNTB

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Product Notes

The FNTB fntb (Catalog #AAA3012706) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FNTB Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FNTB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1,000-1:2,000 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the FNTB fntb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FNTB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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