Rabbit anti-Human FTbeta Polyclonal Antibody | anti-FTbeta antibody

FTbeta (E285) Polyclonal Antibody

Cat. Num. AAA3003689

• Gene Names: FNTB; FPTB
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FTbeta; Polyclonal Antibody; FTbeta (E285) Polyclonal Antibody; Protein farnesyltransferase subunit beta; FTase-beta; FTase beta; CAAX farnesyltransferase subunit beta; Ras proteins prenyltransferase subunit beta; FNTB; anti-FTbeta antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FTbeta (E285) polyclonal antibody detects endogenous levels of FTbeta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 437

• Applicable Applications for anti-FTbeta antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human FTbeta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FTbeta (E285) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:L02 whole cell lysate (40ug))

Related Product Information for anti-FTbeta antibody

Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical alpha and beta subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an a-b heterodimer. The beta subunit, which is known as FTbeta, CAAX farnesyltransferase subunit beta, or Ras proteins prenyltransferase subunit beta, is a 437 amino acid protein that contains five PFTB repeats and binds the peptide substrate. The alpha subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate.

NCBI and Uniprot Product Information

• NCBI GI #: 10835059
• NCBI GeneID: 2342
• NCBI Accession #: NP_002019.1
• NCBI GenBank Nucleotide #: NP_002019.1
• UniProt Accession #: P49356
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: protein farnesyltransferase subunit beta
• NCBI Official Synonym Full Names: farnesyltransferase, CAAX box, beta
• NCBI Official Symbol: FNTB
• NCBI Official Synonym Symbols: FPTB
• NCBI Protein Information: protein farnesyltransferase subunit beta
• UniProt Protein Name: Protein farnesyltransferase subunit beta
• UniProt Gene Name: FNTB
• UniProt Synonym Gene Names: FTase-beta
• UniProt Entry Name: FNTB_HUMAN

Uniprot Description

FNTB: a subunit of farnesyltransferase that is responsible for peptide-binding. Farnesyltransferase catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins.

Protein type: Transferase; EC 2.5.1.58

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: microtubule associated complex; cytosol

Molecular Function: protein binding; farnesyltranstransferase activity; isoprenoid binding; zinc ion binding; drug binding; peptide binding; protein farnesyltransferase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; protein farnesylation; response to inorganic substance; regulation of rhodopsin mediated signaling; wound healing; response to cytokine stimulus; positive regulation of cell cycle; response to organic cyclic substance; positive regulation of nitric-oxide synthase biosynthetic process