Rabbit FLIZ Polyclonal Antibody | anti-FLIZ antibody

Rabbit anti-Escherichia coli (strain K12) FLIZ Polyclonal Antibody

• Gene Names: fliZ; ECK1920; JW1906; yedH
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: FLIZ; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) FLIZ Polyclonal Antibody; FLIZ Antibody; Regulator of sigma S factor FliZ fliZ yedH b1921 JW1906; anti-FLIZ antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-FLIZ antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) fliZ protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 90111356
• NCBI GeneID: 946833
• NCBI Accession #: NP_416431.2
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P52627; P76317
• Molecular Weight: 21,658 Da
• NCBI Official Full Name: RpoS antagonist; putative regulator of FliA activity
• NCBI Official Symbol: fliZ
• NCBI Official Synonym Symbols: ECK1920; JW1906; yedH
• NCBI Protein Information: RpoS antagonist; putative regulator of FliA activity
• UniProt Protein Name: Regulator of sigma S factor FliZ
• Protein Family: Flagellar protein
• UniProt Gene Name: fliZ
• UniProt Synonym Gene Names: yedH

NCBI Description

FliZ antagonizes Crl activation of a subset of RpoS-dependent promoters, without changing RpoS levels, and thus resembles an anti-sigma factor at Crl-stimulated RpoS promoters (Pesavento, 2008). [More information is available at EcoGene: EG11356]. FliZ is an inhibitor of curli expression by reducing the expression of MlrA, YdaM, and YciR. [More information is available at EcoCyc: EG11356].

Uniprot Description

During the post-exponential growth phase transiently interferes with RpoS (sigma S) activity without affecting expression of RpoS itself. It is probably not an anti-sigma factor as its overexpression is detrimental in rapidly growing cells where there is almost no sigma S factor. There is a strong overlap between Crl-activated genes and FliZ-down-regulated genes. FliZ acts as a timing device for expression of the genes for the adhesive curli fimbriae by indirectly decreasing expression of the curli regulator CsgD.