Monkey Complement Component 4 ELISA Kit | C4 elisa kit

Monkey Complement Component 4 ELISA Kit

Cat. Num. AAA761629

• Gene Names: C4BPA; PRP; C4BP
• Reactivity: Monkey
• Synonyms: Complement Component 4; Monkey Complement Component 4 ELISA Kit; C4 elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of C4. No significant crossreactivity or interference between C4 and analogues was observed.
• Sequence Length: 597

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.469-30ng/ml
• Sensitivity: <0.281ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119613909
• NCBI GeneID: 722
• Molecular Weight: 67,033 Da
• NCBI Official Full Name: complement component 4 binding protein, alpha, isoform CRA_c
• NCBI Official Synonym Full Names: complement component 4 binding protein, alpha
• NCBI Official Symbol: C4BPA
• NCBI Official Synonym Symbols: PRP; C4BP
• NCBI Protein Information: C4b-binding protein alpha chain
• UniProt Protein Name: C4b-binding protein alpha chain
• Protein Family: Complement C4
• UniProt Gene Name: C4BPA
• UniProt Synonym Gene Names: C4BP; C4bp; PRP
• UniProt Entry Name: C4BPA_HUMAN

NCBI Description

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: protein binding

Biological Process: complement activation, classical pathway; innate immune response; negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation