Rabbit anti-Human FLASH Polyclonal Antibody | anti-CASP8AP2 antibody

FLASH, CT (FLICE-associated Huge Protein)

Cat. Num. AAA614634

• Gene Names: CASP8AP2; CED-4; FLASH; RIP25; FLJ11208; KIAA1315
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Immunoaffinity chromatography purified IgG
• Synonyms: FLASH; Polyclonal Antibody; CT (FLICE-associated Huge Protein); Anti -FLASH; anti-CASP8AP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FLASH. An approximate 220kD band can be detected.
• Purity/Purification: Affinity Purified; Immunoaffinity chromatography purified IgG
• Form/Format: Supplied as a liquid, 100ug in 200ul of PBS, 0.02% sodium azide.

• Applicable Applications for anti-CASP8AP2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blotting.; Dilution: Western Blotting: 0.25-1ug/ml.
• Immunogen: Raised against a synthetic peptide (SERFQQLMKLFEKSKC) corresponding to amino acids 1253 to 1268 of human origin, which differ from those of mouse by one amino acid (1,2).
• Positive Control: HeLa whole cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemicalanalysis of MBS614634.)

Western Blot (WB)

(Western Blot analysis of MBS614634.)

Related Product Information for anti-CASP8AP2 antibody

A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). FLASH is widely expressed.

Product Categories/Family for anti-CASP8AP2 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 6090856
• NCBI GeneID: 9994
• UniProt Accession #: Q9UKL3; Q6PH76; Q7LCQ7; Q86YD9; Q9NUQ4; Q9NZV9; Q9P2N1; Q9Y563; A2RUB7; E1P553; Q5T791; Q5T792
• Molecular Weight: 222,658 Da
• NCBI Official Full Name: FLASH
• NCBI Official Synonym Full Names: caspase 8 associated protein 2
• NCBI Official Symbol: CASP8AP2
• NCBI Official Synonym Symbols: CED-4; FLASH; RIP25; FLJ11208; KIAA1315
• NCBI Protein Information: CASP8-associated protein 2; human FLASH; OTTHUMP00000016860; FLASH homolog RIP25; FLICE associated huge; CASP8 associated protein 2; FLICE-associated huge protein
• UniProt Protein Name: CASP8-associated protein 2
• Protein Family: CASP8-associated protein
• UniProt Gene Name: CASP8AP2
• UniProt Synonym Gene Names: FLASH; KIAA1315; RIP25
• UniProt Entry Name: C8AP2_HUMAN

NCBI Description

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.

Uniprot Description

CASP8AP2: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.

Protein type: Nuclear receptor co-regulator; Apoptosis

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: PML body; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; death receptor binding; SUMO polymer binding; apoptotic protease activator activity; caspase activator activity; transcription corepressor activity

Biological Process: caspase activation; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; transcription, DNA-dependent; cell cycle; signal transduction