Rabbit anti-Human FKBP4 Polyclonal Antibody | anti-FKBP4 antibody

FKBP4 Antibody (Center)

Cat. Num. AAA9203525

• Gene Names: FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FKBP4; Polyclonal Antibody; FKBP4 Antibody (Center); Peptidyl-prolyl cis-trans isomerase FKBP4; PPIase FKBP4; 51 kDa FK506-binding protein; FKBP51; 52 kDa FK506-binding protein; 52 kDa FKBP; FKBP-52; 59 kDa immunophilin; p59; FK506-binding protein 4; FKBP-4; FKBP59; HSP-binding immunophilin; HBI; Immunophilin FKBP52; Rotamase; N-terminally processed; FKBP52; anti-FKBP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FKBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the Central region of human FKBP4.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 186-216
• Sequence Length: 459

• Applicable Applications for anti-FKBP4 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of FKBP4 Antibody (Center) in Hela,MCF7,T47D cell line lysates(35ug/lane).FKBP4(arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(FKBP4 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of FKBP4 (arrow) using rabbit polyclonal FKBP4 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FKBP4 gene.)

Related Product Information for anti-FKBP4 antibody

FKBP4 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy.

Product Categories/Family for anti-FKBP4 antibody

Signal Transduction

References

Tatro,E.T., J Neuroimmune Pharmacol 4 (2), 218-226 (2009)
Ruan,B., Proc. Natl. Acad. Sci. U.S.A. 105 (1), 33-38 (2008)
Cox,M.B., Mol. Endocrinol. 21 (12), 2956-2967 (2007)

NCBI and Uniprot Product Information

• NCBI GI #: 4503729
• NCBI GeneID: 2288
• NCBI Accession #: NP_002005.1
• NCBI GenBank Nucleotide #: NM_002014.3
• UniProt Accession #: Q02790; Q9UCP1; Q9UCV7; D3DUQ1
• Molecular Weight: 51805
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP4
• NCBI Official Synonym Full Names: FK506 binding protein 4, 59kDa
• NCBI Official Symbol: FKBP4
• NCBI Official Synonym Symbols: HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP4
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP4
• UniProt Synonym Gene Names: FKBP52; PPIase FKBP4; FKBP51; 52 kDa FKBP; FKBP-52; p59; FKBP-4; HBI
• UniProt Entry Name: FKBP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

FKBP4: Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

Protein type: Chaperone; EC 5.2.1.8; Isomerase

Chromosomal Location of Human Ortholog: 12p13.33

Cellular Component: nucleoplasm; microtubule; endoplasmic reticulum membrane; mitochondrion; cell soma; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding, bridging; protein binding; FK506 binding; peptidyl-prolyl cis-trans isomerase activity; GTP binding; heat shock protein binding; phosphoprotein binding; tau protein binding; ATP binding; copper-dependent protein binding; glucocorticoid receptor binding

Biological Process: prostate gland development; male sex differentiation; steroid hormone receptor complex assembly; protein complex localization; protein peptidyl-prolyl isomerization; protein folding; negative regulation of microtubule polymerization; androgen receptor signaling pathway; negative regulation of microtubule polymerization or depolymerization; copper ion transport; embryo implantation