rabbit anti-Mouse, Rat BNIP3 Polyclonal Antibody | anti-BNIP3 antibody

BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3)

Cat. Num. AAA610584

• Gene Names: BNIP3; NIP3
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Affinity chromatography.
• Synonyms: BNIP3; Polyclonal Antibody; BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3; 19kD Interacting Protein 3; NIP3); Anti -BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3; anti-BNIP3 antibody

• Host: rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total BNIP3 protein from mouse and rat.
• Purity/Purification: Affinity Purified; Affinity chromatography.
• Form/Format: Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-BNIP3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser60 of BNIP3 from mouse/rat.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of HeLa cells, mock transfected or transfected with mouse BNIP3, CTLL-2, RAW 264.7 and BaF3 cells MBS610584(Rodent Specific).)

Related Product Information for anti-BNIP3 antibody

BNIP3 (Bcl-2/E1B-19kD interacting prote in 3) is a pro-apoptotic mitochondrial protein and Bcl-2 family member that contains a Bcl-2 homology 3 (BH3) domain a nd a carboxyl terminal transmembrane (TM) domain (1-3). While BNIP3 has a predicted molecular weight of about 22 kD, it runs anomalously on SDS-PAGE and includes a band of around 60 kD that may be a dimeric form that is not re- duced (2). BNIP3 associates with anti-apoptotic family members Bcl-2, Bcl-xL, and the adenovirus homologue E1B-1 9kD. BNIP3 is distinct from other Bcl-2 family members t hat contain only the BH3 domain in that the TM domain, and- not the BH3 domain, is required for mitochondrial targetin- g and pro-apoptotic activity (4). In addition to apoptosis, BNIP3 has been implicated in necrosis (5) and autophagy (6,7), although the relationship between BNIP3 and these processes is not fully understood. Increased expression of BNIP3 under hypoxic conditions is mainly regulated by the transcription factor HIF-1 (8-10). Silencing of the BNIP3 promoter by methylation has been observed in several types of cancer cells and may play an important role in their survival (11-14).

Product Categories/Family for anti-BNIP3 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 7669481
• NCBI GeneID: 664
• NCBI Accession #: NP_004043.2
• NCBI GenBank Nucleotide #: NM_004052.2
• UniProt Accession #: Q12983; O14620; Q96GP0; Q53HF4; Q66K24; Q6NVY4
• Molecular Weight: 21,541 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; OTTHUMP00000020752
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BNIP3
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3
• UniProt Entry Name: BNIP3_HUMAN

NCBI Description

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq]

Uniprot Description

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; dendrite; mitochondrial membrane; cytoplasm; nuclear envelope; nucleus; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; GTPase binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; apoptosis; positive regulation of apoptosis; neuron apoptosis; positive regulation of programmed cell death; response to hyperoxia; autophagic cell death; brown fat cell differentiation; response to hypoxia; mitochondrial fragmentation during apoptosis; regulation of mitochondrial membrane permeability; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis