Goat HAMP / Hepcidin Polyclonal Antibody | anti-HAMP antibody

Goat Polyclonal to Human HAMP / Hepcidin

Cat. Num. AAA247094

• Gene Names: HAMP; HEPC; PLTR; HFE2B; LEAP1
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: HAMP / Hepcidin; Polyclonal Antibody; Goat Polyclonal to Human HAMP / Hepcidin; Anti-HAMP / Hepcidin Antibody (N-Terminus) IHC-plus; HAMP; Hepcidin antimicrobial peptide; LEAP-1; LEAP1; HEPC; Hepcidin; HFE2B; PLTR; Putative liver tumor regressor; Human HAMP; anti-HAMP antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan
• Clonality: Polyclonal
• Specificity: Human HAMP / Hepcidin.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 84

• Applicable Applications for anti-HAMP antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 28kDa band in Human Liver lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the litera...
• Target Species: Human
• Immunogen Description: Synthetic peptide TSGSVFPQQTGQLAE-C from the N-terminus (near) of human HAMP / Hepcidin (NP_066998.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset, Panda (80%).
• Immunogen Type: Synthetic peptide
• Immunogen: HAMP / Hepcidin antibody was raised against synthetic peptide TSGSVFPQQTGQLAE-C from the N-terminus (near) of human HAMP / Hepcidin (NP_066998.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset, Panda (80%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-HAMP / Hepcidin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Related Product Information for anti-HAMP antibody

Hepcidin, an human antimicrobial peptide, contains a leader sequence, a proteolysis site and a C-terminal 25 amino acid active peptide. This peptide is most active against gram-positive bacteria, but also inhibits the growth of certain yeast and gram-negative bacteria. HAMP also functions as a signaling molecule involved in the maintenance of iron homeostasis. In conjugation with HFE it seems to regulate both intestinal iron absorption and iron storage in macrophages.

NCBI and Uniprot Product Information

• NCBI GI #: 10863973
• NCBI GeneID: 57817
• NCBI Accession #: NP_066998.1
• NCBI GenBank Nucleotide #: NM_021175.2
• UniProt Accession #: P81172; Q1HE14; Q9BY68
• Molecular Weight: 9,408 Da
• NCBI Official Full Name: hepcidin preproprotein
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: HAMP
• NCBI Official Synonym Symbols: HEPC; PLTR; HFE2B; LEAP1
• NCBI Protein Information: hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
• UniProt Protein Name: Hepcidin
• Protein Family: Hepcidin
• UniProt Gene Name: HAMP
• UniProt Synonym Gene Names: HEPC; LEAP1; LEAP-1; PLTR; Hepc25; Hepc20
• UniProt Entry Name: HEPC_HUMAN

NCBI Description

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Oct 2014]

Uniprot Description

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: apical cortex; extracellular region

Molecular Function: hormone activity

Biological Process: cellular iron ion homeostasis; killing of cells of another organism; defense response to bacterium; immune response; defense response to fungus

Disease: Hemochromatosis, Type 2b