Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Filaggrin 2 (FLG2) Polyclonal Antibody | anti-FLG2 antibody
Polyclonal Antibody to Filaggrin 2 (FLG2)
Gene Names
FLG2; IFPS
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Filaggrin 2 (FLG2); Polyclonal Antibody; Polyclonal Antibody to Filaggrin 2 (FLG2); anti-FLG2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.35mg/ml (varies by lot)
Applicable Applications for anti-FLG2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Western Blot (WB), Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Cross Reactivity
Human
Traits
Liquid
Immunogen
Recombinant FLG2 (Val1915~Arg2143 (Accession # Q5D862)) expressed in E.coli ( MBS2029453)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
filaggrin-2
NCBI Official Synonym Full Names
filaggrin family member 2
NCBI Official Symbol
FLG2
NCBI Official Synonym Symbols
IFPS
NCBI Protein Information
filaggrin-2
UniProt Protein Name
Filaggrin-2
Protein Family
UniProt Gene Name
FLG2
UniProt Synonym Gene Names
IFPS; FLG-2; Ifapsoriasin
NCBI Description
The filaggrin-like protein encoded by this gene is upregulated by calcium, proteolyzed by calpain 1, and is involved in epithelial homeostasis. The encoded protein is required for proper cornification in skin, with defects in this gene being associated with skin diseases. This protein also has a function in skin barrier protection. In fact, in addition to providing a physical barrier, C-terminal fragments of this protein display antimicrobial activity against P. aeruginosa. [provided by RefSeq, Dec 2015]
Uniprot Description
FLG2: Belongs to the S100-fused protein family.
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: cytoplasm; extracellular region; nucleus
Molecular Function: calcium ion binding; structural molecule activity; transition metal ion binding
Biological Process: neutrophil degranulation
Research Articles on FLG2
Similar Products
Product Notes
The FLG2 flg2 (Catalog #AAA2003658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Filaggrin 2 (FLG2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Filaggrin 2 (FLG2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Western Blot (WB), Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the FLG2 flg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Filaggrin 2 (FLG2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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