Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Angiotensin converting enzyme 2 (ACE2) Standard Curve.)
Rat Angiotensin Converting Enzyme 2 (ACE2) ELISA Kit | ACE2 elisa kit
Rat Angiotensin Converting Enzyme 2 (ACE2) ELISA Kit
Reactivity
Rat
Synonyms
Angiotensin Converting Enzyme 2 (ACE2); Rat Angiotensin Converting Enzyme 2 (ACE2) ELISA Kit; ACE2; ACEH; DKFZp434A014; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin converting enzyme-like protein; ACE2 elisa kit
Reactivity
Rat
Specificity
Rat Angiotensin converting enzyme 2 (ACE2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ACE2. No significant cross-reactivity or interference between Rat ACE2 and analogues was observed.
Sequence Length
805
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Angiotensin converting enzyme 2 (ACE2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Angiotensin converting enzyme 2 (ACE2) Standard Curve.)
Related Product Information for ACE2 elisa kit
Description: This Rat Angiotensin converting enzyme 2 (ACE2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACE2 in samples. An antibody specific for ACE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin,or the conversion of angiotensin II to angiotensin. ACE 2 has direct effects on cardiac function, and is expressed predominantly in vascular endothelial cells of the heart and the kidneys.By EST database searching for sequences showing homology to the zinc metalloprotease angiotensin-I converting enzyme (ACE) and by screening a human lymphoma cDNA library, Tipnis et al. (2000) cloned a full-length ACE2 cDNA, which they called ACEH, encoding a deduced 805-amino acid protein that shares approximately 40% identity with the N- and C-terminal domains of ACE. ACE2 contains a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor.
Background: ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin,or the conversion of angiotensin II to angiotensin. ACE 2 has direct effects on cardiac function, and is expressed predominantly in vascular endothelial cells of the heart and the kidneys.By EST database searching for sequences showing homology to the zinc metalloprotease angiotensin-I converting enzyme (ACE) and by screening a human lymphoma cDNA library, Tipnis et al. (2000) cloned a full-length ACE2 cDNA, which they called ACEH, encoding a deduced 805-amino acid protein that shares approximately 40% identity with the N- and C-terminal domains of ACE. ACE2 contains a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
angiotensin-converting enzyme 2
NCBI Official Synonym Full Names
angiotensin I converting enzyme 2
NCBI Official Symbol
Ace2
NCBI Protein Information
angiotensin-converting enzyme 2
UniProt Protein Name
Angiotensin-converting enzyme 2
Protein Family
UniProt Gene Name
Ace2
NCBI Description
enzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels [RGD, Feb 2006]
Uniprot Description
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.
Research Articles on ACE2
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Product Notes
The Rat ACE2 ace2 (Catalog #AAA9711949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711949 ELISA Kit recognizes Rat ACE2. It is sometimes possible for the material contained within the vial of "Angiotensin Converting Enzyme 2 (ACE2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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