Rabbit anti-Human, Mouse FIGN Polyclonal Antibody | anti-FIGN antibody

FIGN, CT (FIGN, Fidgetin) (PE)

Cat. Num. AAA6299296

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FIGN; Polyclonal Antibody; CT (FIGN; Fidgetin) (PE); Fidgetin; anti-FIGN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FIGN antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FIGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 614-643 amino acids from the C-terminal region of human FIGN.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(FIGN Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the FIGN antibody detected the FIGN protein (arrow).)

Related Product Information for anti-FIGN antibody

Fidgetin is a member of the 'meiotic' or subfamily-7 group of ATPases associated with diverse cellular activities (AAA proteins). Fidgetin can interact with itself and this interaction can be abolished by truncating either the N-or C-terminus of the protein. AAA proteins are molecular chaperones that facilitate membrane fusion, proteolysis, peroxisome biogenesis, endosome sorting and meiotic spindle formation.

Product Categories/Family for anti-FIGN antibody

Antibodies; Proteins

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Alkelai, A., et al. Psychopharmacology (Berl.) 206(3):491-499(2009). Trynka, G., et al. Gut 58(8):1078-1083(2009). Hillier, L.W., et al. Nature 434(7034):724-731(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 64084766
• NCBI GeneID: 55137
• NCBI Accession #: NP_060556.2
• NCBI GenBank Nucleotide #: NM_018086.3
• UniProt Accession #: Q5HY92; Q9H6M5; Q9NVZ9; B3KWM0
• Molecular Weight: 82,146 Da
• NCBI Official Full Name: fidgetin isoform 1
• NCBI Official Synonym Full Names: fidgetin, microtubule severing factor
• NCBI Official Symbol: FIGN
• NCBI Protein Information: fidgetin
• UniProt Protein Name: Fidgetin
• Protein Family: Fidgetin
• UniProt Gene Name: FIGN

Uniprot Description

ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, that may lead to the suppression of microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome.