Rabbit anti-Human Fibroblast Growth Factor 23 (FGF23) Polyclonal Antibody | anti-FGF23 antibody

Polyclonal Antibody to Fibroblast Growth Factor 23 (FGF23)

Cat. Num. AAA2002009

• Gene Names: FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Fibroblast Growth Factor 23 (FGF23); Polyclonal Antibody; Polyclonal Antibody to Fibroblast Growth Factor 23 (FGF23); anti-FGF23 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 0.47mg/ml (varies by lot)

• Applicable Applications for anti-FGF23 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western blotting: 0.5-2ug/mL;; Immunohistochemistry: 5-20ug/mL;; Immunocytochemistry: 5-20ug/mL;; Optimal working dilutions must be determined by end user.
• Organism Species: Homo sapiens (Human)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Human
• Immunogen: Recombinant FGF23 (Ala24~Val126) expressed in E.coli ( MBS2010676)
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

Western Blot (WB)

(Western Blot: Sample: Recombinant FGF23, Human.)

Western Blot

(Western Blot; Sample:Lane 1: Human Cartilage lysate;Lane 2: Human Placenta lysate;Lane 3: Mouse Heart lysatePrimary Ab: 2ug/ml Rabbit Anti-Human FGF23 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:)

NCBI and Uniprot Product Information

• NCBI GI #: 10190674
• NCBI GeneID: 8074
• NCBI Accession #: NP_065689.1
• NCBI GenBank Nucleotide #: NM_020638.2
• UniProt Accession #: Q9GZV9; Q4V758
• NCBI Official Full Name: fibroblast growth factor 23
• NCBI Official Synonym Full Names: fibroblast growth factor 23
• NCBI Official Symbol: FGF23
• NCBI Official Synonym Symbols: ADHR; FGFN; HYPF; HPDR2; PHPTC
• NCBI Protein Information: fibroblast growth factor 23
• UniProt Protein Name: Fibroblast growth factor 23
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF23
• UniProt Synonym Gene Names: HYPF; FGF-23

NCBI Description

This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013]

Uniprot Description

FGF23: Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen

Molecular Function: 1-phosphatidylinositol-3-kinase activity; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; type 1 fibroblast growth factor receptor binding

Biological Process: cell differentiation; cellular phosphate ion homeostasis; cellular protein metabolic process; fibroblast growth factor receptor signaling pathway; MAPK cascade; negative regulation of bone mineralization; negative regulation of hormone secretion; negative regulation of osteoblast differentiation; peptidyl-tyrosine phosphorylation; phosphate ion homeostasis; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; positive regulation of GTPase activity; positive regulation of transcription, DNA-templated; post-translational protein modification; regulation of phosphatidylinositol 3-kinase signaling; response to magnesium ion; vitamin D catabolic process

Disease: Hypophosphatemic Rickets, Autosomal Dominant