Rabbit anti-Human, Rat FHIT Polyclonal Antibody

FHIT (Fragile HistidineTriad)

Cat. Num. AAA615870

• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: FHIT; Polyclonal Antibody; FHIT (Fragile HistidineTriad); Anti -FHIT (Fragile HistidineTriad); anti-FHIT antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FHIT at ~17kD. Reactivity has been con rmed by Western Blot using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-DYKDDDDK construct. Species Crossreactivity: Rat. Does not react with mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-FHIT antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 0.1-1.0ug/ml; Western Blot: 0.3-1.0ug/ml; Immunohistochemistry: 0.5-3.0ug/ml; Formalin-fixed, paraffin embedded sections require a Heat Induced Epitope Retrieval step (HIER).
• Immunogen: Full length human FHIT fusion protein with GST (glutathione amino transferase)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-FHIT antibody

Alterations and deletions within the FHIT (Fragile Histidine Triad) gene and concomitant perturbations of FHIT gene expression are strongly linked to the genesis and establishment of human tumors of the lung (1-3), cervix (4,5), breast (6,7), stomach (8), pancreas (9), and other tissues. Originally identi ed by collaborating researchers in the U.S. and Japan (10), the > 500 kbp FHIT gene was shown to span the fragile chromosomal site FRA3b (11) at band 3p14.2. These researchers and subsequent others demonstrated that loss of heterozygousity or homozygous deletion at this locus are characteristics of multiple tumor-derived cell lines including those from colon, stomach, and breast. Further evidence that FHIT acts as a tumor suppressor in normal cells comes from experiments showing that tumorigenesis of cancer cells is inhibited by replacement of the FHIT gene (12). The involvement of other genes within the FRA3B region in tumor suppression has also been suggested (6). In a comparative study of lung cancers from heavy smokers and non-smokers, alterations in the FHIT gene were found at much higher rates (80% vs. 22%) in tumors from heavy smokers (2). Thus, FHIT may be a ready target for carcinogens present in cigarette smoke.

Product Categories/Family for anti-FHIT antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 122056453
• NCBI Official Full Name: FHIT
• Protein Family: Bis(5'-adenosyl)-triphosphatase

NCBI Description

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.

Protein type: Motility/polarity/chemotaxis; Hydrolase; Tumor suppressor; EC 3.6.1.29; DNA replication; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 3p14.2

Cellular Component: mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; hydrolase activity; ubiquitin protein ligase binding; bis(5'-adenosyl)-triphosphatase activity; nucleotide binding; catalytic activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; nucleotide metabolic process; DNA replication; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; purine nucleotide metabolic process